Scholarlink

<p>Biopterin, a pteridine with roles in photoprotection and enzymatic processes, remains poorly quantified in marine phytoplankton due to methodological limitations. In this study we modified and validated an analytical protocol for extracting and quantifying cellular biopterin in marine cyanobacteria using reversed-phase high-performance liquid chromatography with fluorescence detection (rHPLC-FLD). The method was optimized for pH (3–9) and mobile-phase composition, with recovery rates of 99.4–101.7%. The detection limit was 7.84 pmol, and the quantification limit was 26.1 pmol. Matrix spike recovery rate ranged from 89.1 to 96.3%, and the compound was identified by LC-MS/MS as 6-biopterin (C<sub>9</sub>H<sub>11</sub>N<sub>5</sub>O<sub>3</sub>). The method was successfully applied to quantify biopterin in cultures of the marine microalga <i>Synechococcus</i> sp. WH7803 over 14 days. A significant positive correlation was observed between biopterin and cell abundance (R<sup>2</sup> = 0.74, <i>p</i> &lt; 0.01), suggesting biopterin as a potential growth indicator. The protocol provides a precise analytical tool for studying biopterin dynamics in marine microalgae.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Quantification of cellular biopterin during the culture of marine microalgae using reversed-phase HPLC-FLD method

  • 期刊论文

  • 发表日期：

    2026年5月16日

查看全文
  • Kang Mei,
  • Haowen Zhong,
  • Mengqiu Shi,
  • Deli Wang

摘要

Biopterin, a pteridine with roles in photoprotection and enzymatic processes, remains poorly quantified in marine phytoplankton due to methodological limitations. In this study we modified and validated an analytical protocol for extracting and quantifying cellular biopterin in marine cyanobacteria using reversed-phase high-performance liquid chromatography with fluorescence detection (rHPLC-FLD). The method was optimized for pH (3–9) and mobile-phase composition, with recovery rates of 99.4–101.7%. The detection limit was 7.84 pmol, and the quantification limit was 26.1 pmol. Matrix spike recovery rate ranged from 89.1 to 96.3%, and the compound was identified by LC-MS/MS as 6-biopterin (C9H11N5O3). The method was successfully applied to quantify biopterin in cultures of the marine microalga Synechococcus sp. WH7803 over 14 days. A significant positive correlation was observed between biopterin and cell abundance (R2 = 0.74, p < 0.01), suggesting biopterin as a potential growth indicator. The protocol provides a precise analytical tool for studying biopterin dynamics in marine microalgae.