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<p>Cyanobacteria have shown considerable potential for the remediation of saline-alkaline soils; however, the nitrogen-fixing cyanobacterium <i>Nodularia</i>, often found in saline to brackish waters, has not been evaluated for this purpose. In this study, a strain of <i>Nodularia</i> was isolated from Tsado Chaka Salt Lake in Tibet, China, designated as FACHB-3674 and identified through 16S rDNA sequencing. This strain thrives in NaCl concentrations ranging from 100 to 1000&#xa0;mM and withstands salinity levels up to 1000&#xa0;mM NaCl. It also achieved high biomass when cultured with 100&#xa0;mM NaHCO<sub>3</sub> alone or in combination with 100&#xa0;mM NaCl, while maintaining high nitrogenase activity under these conditions. When applied to saline-alkaline soil, <i>Nodularia</i> reduced the pH from 8.34 to 7.57 and significantly (p &lt; 0.05) increased nutrient availability, including available nitrogen, phosphorus, and organic matter after five months. Concurrently, the soil microbial community structure underwent notable changes, with increased abundances of <i>Bacteroidetes</i>, <i>Myxococcota</i>, <i>Bacillota</i>, <i>Planctomycetota</i>, and <i>Pseudomonadota</i>, and a decrease in <i>Actinomycetota</i>. Microbial communities enriched in genes related to glycosyltransfer, nitrogen fixation, and phosphonate utilization enhanced carbon, nitrogen, and phosphorus cycling and utilization. The results suggested that <i>Nodularia</i> sp. FACHB-3674 was exceptionally salt and alkaline tolerant. Application of the strain will reduce salinization levels and improve nutritional conditions through changes in the soil microbial community. These findings provide valuable insights into the potential application of the nitrogen-fixing cyanobacterium <i>Nodularia</i> sp. for saline-alkaline soil remediation.</p>

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Isolation and characterization of a saline-alkaline tolerant cyanobacterium Nodularia sp. with potential for soil remediation

  • 期刊论文

  • 发表日期：

    2025年12月11日

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  • Lingling Zheng,
  • Guiliang Zheng,
  • Tianli Li,
  • Qi Zhang,
  • Yunlu Jia,
  • Jifeng Zhang,
  • Qi Zhou,
  • Jie Li,
  • Lirong Song

摘要

Cyanobacteria have shown considerable potential for the remediation of saline-alkaline soils; however, the nitrogen-fixing cyanobacterium Nodularia, often found in saline to brackish waters, has not been evaluated for this purpose. In this study, a strain of Nodularia was isolated from Tsado Chaka Salt Lake in Tibet, China, designated as FACHB-3674 and identified through 16S rDNA sequencing. This strain thrives in NaCl concentrations ranging from 100 to 1000 mM and withstands salinity levels up to 1000 mM NaCl. It also achieved high biomass when cultured with 100 mM NaHCO3 alone or in combination with 100 mM NaCl, while maintaining high nitrogenase activity under these conditions. When applied to saline-alkaline soil, Nodularia reduced the pH from 8.34 to 7.57 and significantly (p < 0.05) increased nutrient availability, including available nitrogen, phosphorus, and organic matter after five months. Concurrently, the soil microbial community structure underwent notable changes, with increased abundances of Bacteroidetes, Myxococcota, Bacillota, Planctomycetota, and Pseudomonadota, and a decrease in Actinomycetota. Microbial communities enriched in genes related to glycosyltransfer, nitrogen fixation, and phosphonate utilization enhanced carbon, nitrogen, and phosphorus cycling and utilization. The results suggested that Nodularia sp. FACHB-3674 was exceptionally salt and alkaline tolerant. Application of the strain will reduce salinization levels and improve nutritional conditions through changes in the soil microbial community. These findings provide valuable insights into the potential application of the nitrogen-fixing cyanobacterium Nodularia sp. for saline-alkaline soil remediation.