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<p>Lutein has gained global attention in recent decades in the nutraceutical, pharmaceutical, food, and cosmetic industries due to its strong antioxidant and anticancer properties. Microalgae are a promising source of lutein, with contents reaching up to 1.7% of dry biomass. The present study evaluated the impact of heterotrophic, phototrophic, and mixotrophic cultivation modes on biomass production and lutein yield of three promising microalgal species: <i>Tetraselmis indica</i> BDUG001, <i>Chlorella vulgaris</i> BDGUG003, and <i>Picochlorum</i> sp. BDUG100241. Under mixotrophic conditions, <i>T. indica, C. vulgaris</i> and <i>Picochlorum</i> sp. achieved biomass production of 1.85 ± 0.07, 1.86 ± 0.07, and 1.94 ± 0.03&#xa0;g L<sup>−1</sup>, and lutein yields of 2.41 ± 0.00, 2.39 ± 0.01, and 1.64 ± 0.01&#xa0;mg L<sup>−1</sup>, respectively, with supplementation of 7.5&#xa0;g L<sup>−1</sup> sodium acetate for <i>T. indica</i> and <i>Picochlorum</i> sp, and 1&#xa0;g L<sup>−1</sup> glucose for <i>C. vulgaris</i>. Microalgal lutein was further purified, and its antioxidant activity was assessed using DPPH, ABTS, and OH<sup>●</sup> radical scavenging assays. Lutein extracted from <i>T. indica, C. vulgaris</i> and <i>Picochlorum</i> sp. exhibited DPPH radical scavenging activities of 84.69 ± 0.24, 83.46 ± 0.99, and 83.64 ± 4.19%, and ABTS&#xa0;radical scavenging activities of 85.45 ± 0.82, 84.36 ± 0.28, and 85.12 ± 1.15%, respectively. Furthermore, the characterization of lutein was carried out using FTIR, LC-HRMS, and <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy to confirm its structural integrity.</p>

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Production of lutein from various microalgae species using different cultivation methods: Structural characterization and assessment of functional integrity

  • 期刊论文

  • 发表日期：

    2025年8月28日

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  • Udaypal,
  • Rahul Kumar Goswami,
  • Pradeep Verma

摘要

Lutein has gained global attention in recent decades in the nutraceutical, pharmaceutical, food, and cosmetic industries due to its strong antioxidant and anticancer properties. Microalgae are a promising source of lutein, with contents reaching up to 1.7% of dry biomass. The present study evaluated the impact of heterotrophic, phototrophic, and mixotrophic cultivation modes on biomass production and lutein yield of three promising microalgal species: Tetraselmis indica BDUG001, Chlorella vulgaris BDGUG003, and Picochlorum sp. BDUG100241. Under mixotrophic conditions, T. indica, C. vulgaris and Picochlorum sp. achieved biomass production of 1.85 ± 0.07, 1.86 ± 0.07, and 1.94 ± 0.03 g L−1, and lutein yields of 2.41 ± 0.00, 2.39 ± 0.01, and 1.64 ± 0.01 mg L−1, respectively, with supplementation of 7.5 g L−1 sodium acetate for T. indica and Picochlorum sp, and 1 g L−1 glucose for C. vulgaris. Microalgal lutein was further purified, and its antioxidant activity was assessed using DPPH, ABTS, and OH● radical scavenging assays. Lutein extracted from T. indica, C. vulgaris and Picochlorum sp. exhibited DPPH radical scavenging activities of 84.69 ± 0.24, 83.46 ± 0.99, and 83.64 ± 4.19%, and ABTS radical scavenging activities of 85.45 ± 0.82, 84.36 ± 0.28, and 85.12 ± 1.15%, respectively. Furthermore, the characterization of lutein was carried out using FTIR, LC-HRMS, and 1H and 13C NMR spectroscopy to confirm its structural integrity.