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<p>The low biomass yields and high production costs associated with photoautotrophic and heterotrophic microalgal cultures pose significant economic challenges, thereby necessitating the exploration of alternative growth strategies. The xanthophyte <i>Tribonema minus</i> was selected for its ability to accumulate high amounts of lipids and synthesize valuable fatty acids, which makes it a promising candidate for biofuel production under mixotrophic conditions. The study assessed the effects of varying sodium acetate (NaAc) concentrations on the biomass, lipid content, photosynthetic efficiency, and metabolic pathways of <i>T. minus</i>. Results demonstrated that 0.8&#xa0;g L<sup>−1</sup> NaAc significantly increased biomass (2.01&#xa0;g L<sup>−1</sup>) and lipid content (47.7%), representing 2.93- and 1.16-fold increases compared with the control, respectively. Complementing the biochemical data, FTIR, NMR, and XRD analyses showed that NaAc significantly enhanced lipid and carbohydrate accumulation in <i>T. minus</i>. Although photosynthetic efficiency initially declined, it recovered after 10&#xa0;days, with the maximum photochemical efficiency (F<sub>v</sub>/F<sub>m</sub>) remaining above 0.6. Non-targeted metabolomics revealed that NaAc increased fatty acid production, underscoring its positive role in lipid accumulation. These findings indicate that NaAc is an effective carbon source for optimizing <i>T. minus</i> cultivation, offering a cost-efficient approach to boosting microalgal biomass and lipid production for biofuel applications.</p> Graphical abstract <p></p>

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Optimizing organic carbon supplementation for Tribonema minus: Sodium acetate-driven improvements in biomass, lipid yield, and metabolic pathways

  • 期刊论文

  • 发表日期：

    2025年5月31日

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  • Bingbing Dong,
  • Luyun Cai,
  • Yangguang Wang,
  • Xinyu Yuan,
  • Fang Tian,
  • Ran Xu

摘要

The low biomass yields and high production costs associated with photoautotrophic and heterotrophic microalgal cultures pose significant economic challenges, thereby necessitating the exploration of alternative growth strategies. The xanthophyte Tribonema minus was selected for its ability to accumulate high amounts of lipids and synthesize valuable fatty acids, which makes it a promising candidate for biofuel production under mixotrophic conditions. The study assessed the effects of varying sodium acetate (NaAc) concentrations on the biomass, lipid content, photosynthetic efficiency, and metabolic pathways of T. minus. Results demonstrated that 0.8 g L−1 NaAc significantly increased biomass (2.01 g L−1) and lipid content (47.7%), representing 2.93- and 1.16-fold increases compared with the control, respectively. Complementing the biochemical data, FTIR, NMR, and XRD analyses showed that NaAc significantly enhanced lipid and carbohydrate accumulation in T. minus. Although photosynthetic efficiency initially declined, it recovered after 10 days, with the maximum photochemical efficiency (Fv/Fm) remaining above 0.6. Non-targeted metabolomics revealed that NaAc increased fatty acid production, underscoring its positive role in lipid accumulation. These findings indicate that NaAc is an effective carbon source for optimizing T. minus cultivation, offering a cost-efficient approach to boosting microalgal biomass and lipid production for biofuel applications.

Graphical abstract