<p><i>Pyropia kinositae</i>, a recently discovered species in the northern Yellow Sea, could be an ideal species for offshore <i>Pyropia</i> cultivation in northern regions. This study aims to explore the effects of different temperatures and light intensities on the growth and physiological-biochemical characteristics of <i>P. kinositae</i> to determine the optimal conditions for its blade growth. The results showed that under low temperature (LT: 8&#xa0;°C) conditions, high light intensity (25% of natural light) significantly inhibited the growth of <i>P. kinositae</i>; the highest relative growth rate of <i>P. kinositae</i> was observed at 12.5% of natural light intensity. Under high temperatures (HT: 12&#xa0;°C), low light intensity (6.25%, 3.125% of natural light) was more favorable for the growth of <i>P. kinositae</i>, while high light intensity suppresses the maximum quantum yield (F_v/F_m) of <i>P. kinositae</i>. LT was more conducive to the accumulation of chlorophyll <i>a</i> (Chl <i>a</i>), carotenoid (Car), phycoerythrin (PE), and phycocyanin (PC). Under HT conditions, the effect of light intensity on Chl <i>a</i> and Car was insignificant, but low light intensity favored the synthesis of PE and PC. UV-absorbing compounds, mycosporine-like amino acids (MAAs), decreased with decreasing light intensity. In conclusion, temperature and light intensity significantly impacted on the growth and physiological and biochemical characteristics of <i>P. kinositae</i>, with an interactive effect on the accumulation of various photosynthetic pigments. The results provide insights into better cultivation management and improve the productivity of this economical macroalgae.</p>