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<p>Glucose 6-phosphate (G6P) is an essential metabolite in cyanobacteria as it enters various metabolic pathways including glycolysis and pentose phosphate pathway, and can also serve as a precursor for synthesizing several biologically important compounds. A look into glycogen synthesis and the key regulatory enzyme, ADP-glucose pyrophosphorylase, of this pathway under G6P supplementation (100 μM, 300 μM) showed an increase in the activity of the enzyme along with an upsurge in the enzyme’s content and total glycogen concentration in the cyanobacterium <i>Nostoc muscorum</i> Meg 1. An increase in the mRNA and protein contents relating to the enzyme ADP-glucose pyrophosphorylase under G6P influence indicated that this metabolite could stimulate both transcription and translation of this enzyme at the genetic level. Furthermore, in the presence of additional G6P, the total carbohydrate, soluble protein, and lipid contents in the test organism were found to increase, culminating in increased biomass (12.52 % at 100 μM and 18.70 % at 300 μM G6P). The enhanced glycogen and biomass contents in the organism studied could have constructive implications for industrial applications as glycogen-rich cyanobacterial biomass can serve as feedstock for bioethanol production via fermentation. At a concentration of 500 μM, however, a majority of the parameters examined started declining, indicating that beyond a certain concentration, the added metabolite can induce osmotic stress and thus become detrimental to the organism. This is also substantiated by the scanning and transmission electron micrographs which show deleterious changes induced in the organism’s ultrastructure and morphology at 500 μM G6P.</p>

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Glucose 6-phosphate enrichment augments AGPase enzyme activity and biomass production in the cyanobacterium Nostoc muscorum Meg 1

  • 期刊论文

  • 发表日期：

    2025年1月7日

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  • Lanakadaphi R. Chullai,
  • Balakyntiewshisha Lyngdoh Kynshi,
  • Mayashree B. Syiem

摘要

Glucose 6-phosphate (G6P) is an essential metabolite in cyanobacteria as it enters various metabolic pathways including glycolysis and pentose phosphate pathway, and can also serve as a precursor for synthesizing several biologically important compounds. A look into glycogen synthesis and the key regulatory enzyme, ADP-glucose pyrophosphorylase, of this pathway under G6P supplementation (100 μM, 300 μM) showed an increase in the activity of the enzyme along with an upsurge in the enzyme’s content and total glycogen concentration in the cyanobacterium Nostoc muscorum Meg 1. An increase in the mRNA and protein contents relating to the enzyme ADP-glucose pyrophosphorylase under G6P influence indicated that this metabolite could stimulate both transcription and translation of this enzyme at the genetic level. Furthermore, in the presence of additional G6P, the total carbohydrate, soluble protein, and lipid contents in the test organism were found to increase, culminating in increased biomass (12.52 % at 100 μM and 18.70 % at 300 μM G6P). The enhanced glycogen and biomass contents in the organism studied could have constructive implications for industrial applications as glycogen-rich cyanobacterial biomass can serve as feedstock for bioethanol production via fermentation. At a concentration of 500 μM, however, a majority of the parameters examined started declining, indicating that beyond a certain concentration, the added metabolite can induce osmotic stress and thus become detrimental to the organism. This is also substantiated by the scanning and transmission electron micrographs which show deleterious changes induced in the organism’s ultrastructure and morphology at 500 μM G6P.