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<p>A well-performing electrochemical immunosensor was prepared for detecting the tumor biomarker of carcinoembryonic antigen (CEA) by modifying a glassy carbon electrode (GCE) with a nanocomposite comprising gold nanoparticles (AuNPs), coiled carbon nanotubes (CCNTs), and chitosan (Chi) along with bovine serum proteins and CEA antibody. Various electrochemical methods such as cyclic voltammetry (CV) and square wave voltammetry (WV) were utilized for characterization. The substantial surface area and strong conductivity of AuNPs and CCNTs, and easy film-forming nature of Chi, enhanced the effective surface area and conductivity of the CEA-immunosensors to nearly twice that of the GCE before modification. Under the best experimental conditions, the CEA immunosensor showed good reproducibility with a selective linear detection range of 1&#xa0;pg&#xa0;mL<sup>−1</sup>–400&#xa0;ng&#xa0;mL<sup>−1</sup> and a detection limit as low as 1&#xa0;pg&#xa0;mL<sup>−1</sup>. The decrease in current exhibited a strong linear relationship with the logarithmic values of CEA concentration, boasting a correlation coefficient of 0.99379. The developed CEA immunosensor had good performance and the potential for practical application to real samples with good selectivity and immunity to other antigens in the presence of serum.</p> Graphical abstract <p></p>

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Label-free electrochemical immunosensor based on AuNPs/coiled carbon nanotubes/chitosan-nanocomposites for detection of carcinoembryonic antigen

  • 期刊论文

  • 发表日期：

    2025年1月29日

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  • Xueni Zhang,
  • Yuanzhang Su,
  • Debin Ao,
  • Yan Hong,
  • Min Zou,
  • Jie Xie,
  • Qi Zhou,
  • Yuecong Wang,
  • Wei He,
  • Yuanming Chen

摘要

A well-performing electrochemical immunosensor was prepared for detecting the tumor biomarker of carcinoembryonic antigen (CEA) by modifying a glassy carbon electrode (GCE) with a nanocomposite comprising gold nanoparticles (AuNPs), coiled carbon nanotubes (CCNTs), and chitosan (Chi) along with bovine serum proteins and CEA antibody. Various electrochemical methods such as cyclic voltammetry (CV) and square wave voltammetry (WV) were utilized for characterization. The substantial surface area and strong conductivity of AuNPs and CCNTs, and easy film-forming nature of Chi, enhanced the effective surface area and conductivity of the CEA-immunosensors to nearly twice that of the GCE before modification. Under the best experimental conditions, the CEA immunosensor showed good reproducibility with a selective linear detection range of 1 pg mL−1–400 ng mL−1 and a detection limit as low as 1 pg mL−1. The decrease in current exhibited a strong linear relationship with the logarithmic values of CEA concentration, boasting a correlation coefficient of 0.99379. The developed CEA immunosensor had good performance and the potential for practical application to real samples with good selectivity and immunity to other antigens in the presence of serum.

Graphical abstract