Purpose <p>This study conducts a comprehensive bibliometric analysis regarding the application of ultrasound biomicroscopy in glaucoma research over the past two decades.</p> Methods <p>Bibliometric analysis was performed on relevant literature published between 2005 and 2024. Data pertaining to authorship, affiliations, countries of origin, journals, keywords, and cited references were extracted. Visualization and analysis were executed utilizing VOSviewer and CiteSpace software.</p> Results <p>Bibliometric analysis of 557 articles revealed a quadratic growth trend in annual publication volume. The most productive authors, institutions, countries, and journals were identified and collaboration network maps were drawn. Keyword co-occurrence analysis identified five primary research clusters: angle-closure glaucoma pathogenesis, cataract surgery &amp; complications, glaucoma surgery, ciliary body &amp; iris, and iridocorneal angle. Burst detection and timeline analysis highlighted emerging research frontiers, including deep learning, 3D reconstruction, vitreous zonule imaging, and minimally invasive glaucoma surgery. These findings signal a shift in research priorities towards computational approaches and novel clinical applications.</p> Conclusion <p>This bibliometric analysis delineates the dynamic evolution and broadening scope of ultrasound biomicroscopy application in glaucoma research over the past two decades. Collectively, these findings affirm the enduring value of ultrasound biomicroscopy and delineate promising avenues for future investigation.</p>