Scholarlink

<p>The temporal fluctuation in environmental DNA (eDNA) production may influence the sampling timing and effort required to reliably estimate species distribution and abundance using eDNA analysis. To understand how the amount of eDNA released fluctuates within a day on a scale of hours, we conducted tank experiments where common carp (<i>Cyprinus carpio</i>) were reared under different feeding conditions. Over 2 days, water samples were collected every 3 h to estimate the concentrations of its mitochondrial and nuclear eDNA. The amount of eDNA released exhibited temporally nonlinear fluctuation with their peaks observed during the lighting period for the unfed condition, whereas the temporal fluctuations were unclear for the fed condition. Additionally, we found that feeding did not noticeably increase eDNA concentrations or promote their production regardless of the marker types. These results suggest that the relationship between feeding and eDNA production may not be simple, and, without obvious foraging and excretion behaviors, behavioral rhythms and basal metabolism of individuals can potentially be represented as the temporal fluctuation in eDNA production. Our findings contribute to understanding the eDNA production process in relation to an organism's activity and behavior, providing implications of eDNA signals depending on the aim of eDNA application.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Short-term temporal fluctuation in fish environmental DNA production in relation to feeding condition and marker type

  • 期刊论文

  • 发表日期：

    2026年7月29日

查看全文
  • Toshiaki S. Jo,
  • Shiori Ogawa,
  • Kana Hayami,
  • Takeshi Watanabe,
  • Nobutaka Maki,
  • Qianqian Wu,
  • Toshifumi Minamoto

摘要

The temporal fluctuation in environmental DNA (eDNA) production may influence the sampling timing and effort required to reliably estimate species distribution and abundance using eDNA analysis. To understand how the amount of eDNA released fluctuates within a day on a scale of hours, we conducted tank experiments where common carp (Cyprinus carpio) were reared under different feeding conditions. Over 2 days, water samples were collected every 3 h to estimate the concentrations of its mitochondrial and nuclear eDNA. The amount of eDNA released exhibited temporally nonlinear fluctuation with their peaks observed during the lighting period for the unfed condition, whereas the temporal fluctuations were unclear for the fed condition. Additionally, we found that feeding did not noticeably increase eDNA concentrations or promote their production regardless of the marker types. These results suggest that the relationship between feeding and eDNA production may not be simple, and, without obvious foraging and excretion behaviors, behavioral rhythms and basal metabolism of individuals can potentially be represented as the temporal fluctuation in eDNA production. Our findings contribute to understanding the eDNA production process in relation to an organism's activity and behavior, providing implications of eDNA signals depending on the aim of eDNA application.