<p>Although the aquarium trade is an important pathway for direct and indirect non-native species introductions into freshwater systems, knowledge and attitudes of participants in the trade regarding alien species issues is largely undocumented. Therefore, we administered a survey to investigate attitudes and behaviors of aquarists and non-aquarists regarding the aquarium trade as a pathway for invasive species introductions. We hypothesized aquarists would be better educated on potential introductions than non-aquarists. Due to their engagement with organisms and replication of natural habitats, we also expected aquarists would exhibit more positive attitudes and behaviors toward the environment than non-aquarists. Most respondents seemingly understood the severity of invasive species problems, regardless of status as an aquarist. Aquarists thought the aquarium trade was less responsible for species introductions than non-aquarists and also felt more aware of laws regarding invasive species. However, over half of aquarists disposed of aquarium water down a drain in their house or directly into a nearby water body, which is a known mode of invasion. This information can inform further efforts to provide educational opportunities for participants in the non-native pet/aquarium trade, with the goal of enhancing protection efforts for native biodiversity.</p>