Scholarlink

<p>Tooth extraction is a common procedure often followed by alveolar bone resorption, which may compromise future implant placement, prosthetic rehabilitation, esthetics, and periodontal support. Hydroxyapatite–chitosan (HA–Chi) scaffolds combined with human umbilical cord mesenchymal stem cells (hUCMSCs) may enhance bone healing by modulating bone remodeling pathways. This study evaluated the effects of hUCMSC-seeded HA–Chi scaffolds on osteoprotegerin (OPG) and receptor activator of nuclear factor-κB (RANK) expression during alveolar socket healing. In this randomized controlled in vivo experiment, 45 male Wistar rats underwent mandibular incisor extraction and were allocated to three groups: untreated control (C), HA–Chi scaffold only (T1), and hUCMSC-seeded HA–Chi scaffold (T2). Each group included 15 animals, with five evaluated at 7, 14, and 28 days. OPG and RANK expression were assessed immunohistochemically and data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. OPG expression increased progressively over time in all groups, whereas RANK expression decreased. The hUCMSC-seeded HA–Chi scaffold group consistently showed the highest OPG and lowest RANK expression at all observation periods. Overall intergroup differences were significant for both markers (<i>p</i> &lt; 0.001). For OPG, no significant difference was observed between T1 and T2 at day 7, whereas significant differences emerged at days 14 and 28. For RANK, the hUCMSC-seeded group showed significantly lower expression than the control group at all-time points and than the scaffold-only group at day 28. hUCMSC-seeded HA–Chi scaffolds promoted a favorable bone remodeling profile by upregulated OPG and downregulated RANK, indicating strong potential for alveolar socket preservation after tooth extraction.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Alveolar socket preservation with human umbilical cord mesenchymal stem cell-seeded hydroxyapatite–chitosan scaffolds: An in vivo assessment of osteoprotegerin and receptor activator of nuclear factor-κB expression

  • 期刊论文

  • 发表日期：

    2026年6月25日

查看全文
  • Dhafer Abdulwasea Alshaibani,
  • Michael Josef Kamadjaja,
  • Ratri Maya Sitalaksmi,
  • Dwi Wahyu Indrawati,
  • Darmawan Setijanto,
  • Sultan Ainoosah,
  • Ahmed Yaseen Alqutaibi,
  • Redhwan Saleh Al-Gabri

摘要

Tooth extraction is a common procedure often followed by alveolar bone resorption, which may compromise future implant placement, prosthetic rehabilitation, esthetics, and periodontal support. Hydroxyapatite–chitosan (HA–Chi) scaffolds combined with human umbilical cord mesenchymal stem cells (hUCMSCs) may enhance bone healing by modulating bone remodeling pathways. This study evaluated the effects of hUCMSC-seeded HA–Chi scaffolds on osteoprotegerin (OPG) and receptor activator of nuclear factor-κB (RANK) expression during alveolar socket healing. In this randomized controlled in vivo experiment, 45 male Wistar rats underwent mandibular incisor extraction and were allocated to three groups: untreated control (C), HA–Chi scaffold only (T1), and hUCMSC-seeded HA–Chi scaffold (T2). Each group included 15 animals, with five evaluated at 7, 14, and 28 days. OPG and RANK expression were assessed immunohistochemically and data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. OPG expression increased progressively over time in all groups, whereas RANK expression decreased. The hUCMSC-seeded HA–Chi scaffold group consistently showed the highest OPG and lowest RANK expression at all observation periods. Overall intergroup differences were significant for both markers (p < 0.001). For OPG, no significant difference was observed between T1 and T2 at day 7, whereas significant differences emerged at days 14 and 28. For RANK, the hUCMSC-seeded group showed significantly lower expression than the control group at all-time points and than the scaffold-only group at day 28. hUCMSC-seeded HA–Chi scaffolds promoted a favorable bone remodeling profile by upregulated OPG and downregulated RANK, indicating strong potential for alveolar socket preservation after tooth extraction.