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<p>Balsa (<i>Ochroma pyramidale</i>) is a fast-growing tree species with extremely high economic value, but its seedling propagation is limited by inefficient traditional propagation methods. In this study, we established an efficient and stable in vitro regeneration and micropropagation system for balsa and characterized the gene expression patterns associated with shoot and root regeneration. By systematically comparing the regenerative capacities of different explants, we identified the optimal explant and the best phytohormone combinations for shoot regeneration and for root regeneration. B3 medium was optimal for inducing multiple shoots, and R2 medium achieved a rooting rate up to 90% ± 1.37%. Pretreatment of seedlings with the H1 medium promoted stem thickening, enabling efficient regeneration of complete plants from explants. Furthermore, by identifying homologous genes involved in shoot and root regeneration in balsa and combining this with transcriptomic and RT–qPCR analyses, we revealed the expression patterns and characteristics of potential regulatory genes for regeneration processes. Based on these results, we propose a preliminary regulatory framework for shoot and root regeneration in balsa. Our findings provided a reliable technical pathway for the large-scale propagation of this important economic tree species and laid a potential molecular foundation for further regulating the growth and development and wood traits of balsa through gene editing and other means.</p>

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Efficient in vitro regeneration and gene regulation of shoot and root organogenesis in Ochroma pyramidale

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月22日

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  • Jiaxin Wang,
  • Haijiao Li,
  • Siyuan Wu,
  • Zhiyu Chen,
  • Minghui Chen,
  • Yifan Cai,
  • Tianping Huang,
  • Xin Yin,
  • Yan Zheng,
  • Yongping Yang,
  • Yunqiang Yang,
  • Xiangxiang Kong

摘要

Balsa (Ochroma pyramidale) is a fast-growing tree species with extremely high economic value, but its seedling propagation is limited by inefficient traditional propagation methods. In this study, we established an efficient and stable in vitro regeneration and micropropagation system for balsa and characterized the gene expression patterns associated with shoot and root regeneration. By systematically comparing the regenerative capacities of different explants, we identified the optimal explant and the best phytohormone combinations for shoot regeneration and for root regeneration. B3 medium was optimal for inducing multiple shoots, and R2 medium achieved a rooting rate up to 90% ± 1.37%. Pretreatment of seedlings with the H1 medium promoted stem thickening, enabling efficient regeneration of complete plants from explants. Furthermore, by identifying homologous genes involved in shoot and root regeneration in balsa and combining this with transcriptomic and RT–qPCR analyses, we revealed the expression patterns and characteristics of potential regulatory genes for regeneration processes. Based on these results, we propose a preliminary regulatory framework for shoot and root regeneration in balsa. Our findings provided a reliable technical pathway for the large-scale propagation of this important economic tree species and laid a potential molecular foundation for further regulating the growth and development and wood traits of balsa through gene editing and other means.