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<p>Environmental&#xa0;drought inhibits plant growth and yield. As a fructan accumulating tuber crop member of the Asteraceae family, Jerusalem artichoke (<i>Helianthus tuberosus</i> L.) has excellent resistance to stress. A morphological and physiological comparison of Jerusalem artichoke genotypes QY1 and QY3 was assessed to distinguish their differential responses to drought stress. To reveal the molecular mechanisms underlying these responses, we compared the transcriptome between these two genotypes under both well-watered and drought conditions. A gene co-expression network was constructed using the weighted gene co-expression network analysis method, and the core genes were further tested by performing genetic loss-of-function studies. Significant differences in root water content, chlorophyll content, superoxide dismutase activity, and carbohydrate levels were observed between QY1 and QY3 genotypes under drought conditions, with QY3 showing superior drought adaptation. Both the morphological and physiological characteristics showed significant differences between the two Jerusalem artichoke genotypes, which was further supported by significant differences in gene expression related to secondary metabolism, light reaction, and cell wall processes. Four key gene modules were identified as having strong associations with drought stress traits. Based on functional annotation of differential genes, we selected 16 key genes from these four modules. Our results demonstrate that Arabidopsis mutants with knockout mutations in genes encoding phospholipid/glycerol acyltransferase and protein kinase with ATP binding sites exhibited decreased soluble sugar and proline contents, resulting in drought-sensitive phenotypes. New drought stress-responsive genes were identified and the drought-regulated mechanisms underlying the differential responses of QY1 and QY3 Jerusalem artichoke genotypes were elucidated. This extends the stress response previously observed for some non-model species and provides new insight into Jerusalem artichoke drought stress-responsive genes.</p>

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A comprehensive analysis of transcriptome and weighted gene co-expression network (WGCNA) reveals functional genes participating in drought stress response of Jerusalem artichoke (Helianthus tuberosus L.)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月4日

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  • Lihui Wang,
  • Cheng Si,
  • Shipeng Yang,
  • Qiwen Zhong,
  • Guangnan Zhang,
  • Haiwang Zhang,
  • Dengshan Zhang,
  • Xuemei Sun

摘要

Environmental drought inhibits plant growth and yield. As a fructan accumulating tuber crop member of the Asteraceae family, Jerusalem artichoke (Helianthus tuberosus L.) has excellent resistance to stress. A morphological and physiological comparison of Jerusalem artichoke genotypes QY1 and QY3 was assessed to distinguish their differential responses to drought stress. To reveal the molecular mechanisms underlying these responses, we compared the transcriptome between these two genotypes under both well-watered and drought conditions. A gene co-expression network was constructed using the weighted gene co-expression network analysis method, and the core genes were further tested by performing genetic loss-of-function studies. Significant differences in root water content, chlorophyll content, superoxide dismutase activity, and carbohydrate levels were observed between QY1 and QY3 genotypes under drought conditions, with QY3 showing superior drought adaptation. Both the morphological and physiological characteristics showed significant differences between the two Jerusalem artichoke genotypes, which was further supported by significant differences in gene expression related to secondary metabolism, light reaction, and cell wall processes. Four key gene modules were identified as having strong associations with drought stress traits. Based on functional annotation of differential genes, we selected 16 key genes from these four modules. Our results demonstrate that Arabidopsis mutants with knockout mutations in genes encoding phospholipid/glycerol acyltransferase and protein kinase with ATP binding sites exhibited decreased soluble sugar and proline contents, resulting in drought-sensitive phenotypes. New drought stress-responsive genes were identified and the drought-regulated mechanisms underlying the differential responses of QY1 and QY3 Jerusalem artichoke genotypes were elucidated. This extends the stress response previously observed for some non-model species and provides new insight into Jerusalem artichoke drought stress-responsive genes.