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<p>Fusarium wilt of banana is one of the debilitating diseases of banana, which is caused by a fungal pathogen <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc). Conventional management strategies to control Fusarium wilt have not provided adequate solution. It is essential to develop an environment-friendly, economically viable ready deployable solution to reduce disease severity in banana plants. The present study aimed to isolate rhizobacteria from banana fields that possess antifungal activity against Foc. A total of six rhizobacterial isolates (SVS01-06) were identified that demonstrated anti-Foc activity with inhibition ranging from 10 to 22%. The 16s rRNA gene sequencing revealed that these rhizobacterial isolates belonged to <i>Bacillus</i> and <i>Paenibacillus</i> genera. Further, these isolates were investigated for their in vitro plant growth promoting abilities and demonstrated traits such as phosphate mineralization, biofilm formation, production of hydrolytic enzymes, hydrogen cyanide, acetoin, ammonia, indole acetic acid and inhibitory volatile organic compounds. These isolates were non-hemolytic and compatible with each other. Gas Chromatography-Mass Spectrometry analyses revealed the presence of inhibitory volatile compounds such as heptacosane, pentadecanal, dimethyl undecane, undecane, and 2-isopropyl-5-methyl-1-heptanol produced by these isolates. No significant growth benefit was offered by the rhizobacterial isolates to the banana plants under controlled conditions, but displayed protection to banana root segments when treated with Foc. More than 50% reduction in the Fusarium wilt disease symptoms was observed in banana plants treated with these isolates, especially by SVS02, under greenhouse conditions. These results revealed that the rhizobacterial isolates, and SVS02 in particular, possesses immense potential to improve plant growth under stress conditions in addition to suppressing Fusarium wilt disease in banana.</p>

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Control of Fusarium wilt disease of banana using native rhizospheric bacteria

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月4日

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  • Shalu Pankaj,
  • Janhavi D. More,
  • Aparna Tiwari,
  • Aruljyothi Paramasivam,
  • Siddhesh B. Ghag

摘要

Fusarium wilt of banana is one of the debilitating diseases of banana, which is caused by a fungal pathogen Fusarium oxysporum f. sp. cubense (Foc). Conventional management strategies to control Fusarium wilt have not provided adequate solution. It is essential to develop an environment-friendly, economically viable ready deployable solution to reduce disease severity in banana plants. The present study aimed to isolate rhizobacteria from banana fields that possess antifungal activity against Foc. A total of six rhizobacterial isolates (SVS01-06) were identified that demonstrated anti-Foc activity with inhibition ranging from 10 to 22%. The 16s rRNA gene sequencing revealed that these rhizobacterial isolates belonged to Bacillus and Paenibacillus genera. Further, these isolates were investigated for their in vitro plant growth promoting abilities and demonstrated traits such as phosphate mineralization, biofilm formation, production of hydrolytic enzymes, hydrogen cyanide, acetoin, ammonia, indole acetic acid and inhibitory volatile organic compounds. These isolates were non-hemolytic and compatible with each other. Gas Chromatography-Mass Spectrometry analyses revealed the presence of inhibitory volatile compounds such as heptacosane, pentadecanal, dimethyl undecane, undecane, and 2-isopropyl-5-methyl-1-heptanol produced by these isolates. No significant growth benefit was offered by the rhizobacterial isolates to the banana plants under controlled conditions, but displayed protection to banana root segments when treated with Foc. More than 50% reduction in the Fusarium wilt disease symptoms was observed in banana plants treated with these isolates, especially by SVS02, under greenhouse conditions. These results revealed that the rhizobacterial isolates, and SVS02 in particular, possesses immense potential to improve plant growth under stress conditions in addition to suppressing Fusarium wilt disease in banana.