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<p>DNA barcoding is an effective approach for species identification and phylogenetic inference, particularly in taxonomically complex genera such as <i>Artemisia</i>. In this study, four Turkish species (<i>Artemisia incana</i>, <i>A. spicigera</i>, <i>A. splendens</i>, and <i>A. taurica</i>) were molecularly characterized using the chloroplast <i>matK</i> region, and their sequences were deposited in public barcode databases. Genomic DNA was successfully extracted and quantified, yielding high-quality templates for PCR amplification. The <i>matK</i> region (~ 750&#xa0;bp) was amplified and sequenced, and the obtained sequences were compared with 116 reference sequences retrieved from GenBank. Phylogenetic analyses based on maximum likelihood, Bayesian inference, and neighbor-joining methods consistently revealed that <i>A. splendens</i> and <i>A. taurica</i> formed distinct clades with moderate divergence, whereas <i>A. incana</i> and <i>A. spicigera</i> clustered together with strong statistical support. Codon usage analysis indicated a typical AT-rich composition of chloroplast genes, and model testing identified the T92 + I model as the best fit for nucleotide substitution. Despite its effectiveness, the <i>matK</i> marker showed limited resolution in distinguishing closely related species, suggesting that the inclusion of additional loci may improve species discrimination. This study provides the first <i>matK</i>-based DNA barcode data for these four Turkish <i>Artemisia</i> species, contributing valuable molecular resources for taxonomy, phylogenetics, and conservation research within the genus.</p>

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Molecular characterization of some Artemisia Species using DNA barcoding and contribution to the DNA barcode database

  • 期刊论文

  • 发表日期：

    2026年6月15日

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  • Hacı Furkan Küçükgöl,
  • Dilara Bakmaz,
  • Ahmed Sidar Aygören,
  • Emre İlhan,
  • Murat Kürşat,
  • Ali Rıza Tüfekçi,
  • Ebru Derelli Tüfekçi

摘要

DNA barcoding is an effective approach for species identification and phylogenetic inference, particularly in taxonomically complex genera such as Artemisia. In this study, four Turkish species (Artemisia incana, A. spicigera, A. splendens, and A. taurica) were molecularly characterized using the chloroplast matK region, and their sequences were deposited in public barcode databases. Genomic DNA was successfully extracted and quantified, yielding high-quality templates for PCR amplification. The matK region (~ 750 bp) was amplified and sequenced, and the obtained sequences were compared with 116 reference sequences retrieved from GenBank. Phylogenetic analyses based on maximum likelihood, Bayesian inference, and neighbor-joining methods consistently revealed that A. splendens and A. taurica formed distinct clades with moderate divergence, whereas A. incana and A. spicigera clustered together with strong statistical support. Codon usage analysis indicated a typical AT-rich composition of chloroplast genes, and model testing identified the T92 + I model as the best fit for nucleotide substitution. Despite its effectiveness, the matK marker showed limited resolution in distinguishing closely related species, suggesting that the inclusion of additional loci may improve species discrimination. This study provides the first matK-based DNA barcode data for these four Turkish Artemisia species, contributing valuable molecular resources for taxonomy, phylogenetics, and conservation research within the genus.