<p>Soil salinity significantly reduces the yield and quality of bread wheat (<i>Triticum aestivum</i> L.), while the phytohormone ethylene is well known to modulate plant responses to salt tolerance. Acyltransferases play diverse roles in plants, including the improvement of salt tolerance. In this study, we identified and characterized acyltransferase genes associated with ethylene signaling and salt stress responses in bread wheat. A total of 68 acyltransferase genes were identified, including 17 expressed in the root, 52 in the shoot, and 1 in both tissues, suggesting tissue-specific expression and functional diversity. These 68 acyltransferase genes were further analyzed for phylogenic relationships, gene structures, chromosomal locations, conserved domains, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and expression patterns. The results showed that the acyltransferase genes were classified into 5 major groups, and unevenly distributed across most chromosomes. The acyltransferase genes potentially played important roles in glycerophospholipid metabolism, glycerolipid metabolism, other metabolic pathways, and the biosynthesis of secondary metabolites related to anthocyanin biosynthesis, membrane constructions, and components involved in ethylene and salt responses of bread wheat. Among them, <i>DCR-3</i> was selected based on expression profiles. Overexpression of <i>DCR-3</i> in <i>Arabidopsis</i> reduced salt tolerance, while CRISPR/Cas9-mediated deletion of <i>DCR-3</i> in wheat enhanced salt tolerance. The study provides a strong basis for further functional investigation on the wheat acyltransferase genes in ethylene-responsive salt responses. The 68 identified acyltransferase genes present valuable sources for salt-tolerance breeding in wheat and may serve as key links between ethylene signaling and salt tolerance in higher plants.</p>