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<p><i>Basella alba</i> L. (Basellaceae) is a popular leafy vegetable in the hot humid tropics of India, tropical Asia and Africa. The species is represented by two cultivated colour-morphs – green stemmed and red-stemmed and one crop-wild twining variant with habitual and morphological variability. Such variability has favoured the introduction of three varieties under <i>Basella alba</i> L. viz.- var. <i>alba</i>, var. <i>rubra</i> and var. <i>scandens</i>. Phylogenetic linkage among the varieties has been traced applying DNA barcoding of internal transcribed spacer (ITS) and&#xa0;the large subunit of Ribulose 1,5-bisphosphate Carboxylase/oxygenase encoding (<i>rbcL</i>) gene&#xa0;sequences as well as inter-simple sequence repeat (ISSR) polymorphism. Resultant ITS sequences showed extreme polymorphism but <i>rbcL</i> sequences appeared with high level of sequence uniformity. Phylogenetic tree constructed on ITS sequences clearly reflected separate molecular identity of the varieties, inclusion of cultivated varieties with close ally <i>Anredera cordifolia</i> in a single clade and crop-wild <i>Basella alba</i> var. <i>scandens</i> as probable progenitor of two cultivated varieties in separate lineage. Phylogenetic tree computed on <i>rbcL</i> sequences showed conspecific nature of the varieties, there inclusion in a single clade, so as the allied species of <i>Anredera</i>. Dendrogram on ISSR polymorphism also consolidated separate identity of the varieties as well as close alliance of crop-wild variety with red-stemmed cultivated form as the probable immediate descendent. The study strongly favoured infraspecific classification of <i>Basella alba</i> comprising three varieties rather than treating them as separate species.</p>

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Molecular delimitation and phylogeny of Basella alba L. varieties applying DNA barcoding and inter-simple sequence repeat (ISSR) polymorphism

  • 期刊论文

  • 发表日期：

    2026年6月2日

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  • Saubhik Das,
  • Sagnik Das

摘要

Basella alba L. (Basellaceae) is a popular leafy vegetable in the hot humid tropics of India, tropical Asia and Africa. The species is represented by two cultivated colour-morphs – green stemmed and red-stemmed and one crop-wild twining variant with habitual and morphological variability. Such variability has favoured the introduction of three varieties under Basella alba L. viz.- var. alba, var. rubra and var. scandens. Phylogenetic linkage among the varieties has been traced applying DNA barcoding of internal transcribed spacer (ITS) and the large subunit of Ribulose 1,5-bisphosphate Carboxylase/oxygenase encoding (rbcL) gene sequences as well as inter-simple sequence repeat (ISSR) polymorphism. Resultant ITS sequences showed extreme polymorphism but rbcL sequences appeared with high level of sequence uniformity. Phylogenetic tree constructed on ITS sequences clearly reflected separate molecular identity of the varieties, inclusion of cultivated varieties with close ally Anredera cordifolia in a single clade and crop-wild Basella alba var. scandens as probable progenitor of two cultivated varieties in separate lineage. Phylogenetic tree computed on rbcL sequences showed conspecific nature of the varieties, there inclusion in a single clade, so as the allied species of Anredera. Dendrogram on ISSR polymorphism also consolidated separate identity of the varieties as well as close alliance of crop-wild variety with red-stemmed cultivated form as the probable immediate descendent. The study strongly favoured infraspecific classification of Basella alba comprising three varieties rather than treating them as separate species.