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<p><i>EIN3/EILs</i> transcription factors are key regulators in the plant ethylene signaling pathway. They play a role in defending against both biotic and abiotic stresses, as well as responding to iron and sulfur deficiency in plants. However, there is still a knowledge gap regarding the regulatory mechanism of the EIL gene family in oat. In this study, a genome-wide characterization in oat led to the identification of 22 <i>AsEIL</i> genes, all of which were found to be localized in the nucleus. These AsEIL genes were evenly distributed among subgenomes A, C, and D, but only appeared on 11 out of the 21 chromosomes,and were absent from chromosomes 1A/1C/1D/3A/3C/and 3D. Phylogenetic analysis classified the 22 <i>AsEILs</i> into three subfamilies, with the highest number of <i>AsEILs</i> belonging to Group A. Additionally, all <i>AsEIL</i> proteins contained Motif 1 and Motif 4. The promoter region of these genes contained various cis-acting elements related to light, hormones, and abiotic stresses. Collinearity analysis revealed that segmental duplication was the primary mode of expansion of the AsEIL gene family. Oat <i>AsEIL</i> showed the highest homology with wheat <i>TaEIL</i>, while were distantly related to Arabidopsis EILs was found with <i>Arabidopsis EIL</i> genes. RT-qPCR analysis indicated that the expression of <i>AsEIL002/007/010</i> was significantly up-regulated in both oat cultivars at 24&#xa0;h after salt stress and iron stress. This suggests that these three genes may play a crucial regulatory role in the adaptation to iron and salt stress. Furthermore, the regulatory mechanisms involved in adversity stress and nutrient uptake warrant further investigation.</p>

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Genome-wide identification of oat EIL gene family and analysis of expression patterns under abiotic stress

  • 期刊论文

  • 发表日期：

    2026年3月10日

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  • Xiaodong Guo,
  • Xuanyu Liu,
  • Jiaming Nie,
  • Qidi Zhang,
  • Simeng Cai,
  • Huiyan Liu,
  • Bing Han,
  • Hongbin Zhao

摘要

EIN3/EILs transcription factors are key regulators in the plant ethylene signaling pathway. They play a role in defending against both biotic and abiotic stresses, as well as responding to iron and sulfur deficiency in plants. However, there is still a knowledge gap regarding the regulatory mechanism of the EIL gene family in oat. In this study, a genome-wide characterization in oat led to the identification of 22 AsEIL genes, all of which were found to be localized in the nucleus. These AsEIL genes were evenly distributed among subgenomes A, C, and D, but only appeared on 11 out of the 21 chromosomes,and were absent from chromosomes 1A/1C/1D/3A/3C/and 3D. Phylogenetic analysis classified the 22 AsEILs into three subfamilies, with the highest number of AsEILs belonging to Group A. Additionally, all AsEIL proteins contained Motif 1 and Motif 4. The promoter region of these genes contained various cis-acting elements related to light, hormones, and abiotic stresses. Collinearity analysis revealed that segmental duplication was the primary mode of expansion of the AsEIL gene family. Oat AsEIL showed the highest homology with wheat TaEIL, while were distantly related to Arabidopsis EILs was found with Arabidopsis EIL genes. RT-qPCR analysis indicated that the expression of AsEIL002/007/010 was significantly up-regulated in both oat cultivars at 24 h after salt stress and iron stress. This suggests that these three genes may play a crucial regulatory role in the adaptation to iron and salt stress. Furthermore, the regulatory mechanisms involved in adversity stress and nutrient uptake warrant further investigation.