Scholarlink

<p>This study evaluated the genetic and phytochemical diversity of 35 <i>Cymbopogon</i> accessions from Vietnam and Laos using an integrated approach combining <i>ITS</i> sequencing, random amplified polymorphic DNA (RAPD) markers, and GC–MS profiling. <i>ITS</i> analysis identified 33 accessions as <i>Cymbopogon citratus</i> and two as <i>Cymbopogon winterianus</i>, revealing only two major haplotypes and indicating low intraspecific variation, likely due to clonal propagation. RAPD analysis using eight highly polymorphic primers generated 86 bands with 61.28% polymorphism, indicating moderate genetic diversity and forming two distinct clusters corresponding to two species boundaries. The lack of clear geographic structuring suggests extensive exchange of planting materials, resulting in the genetic homogenization of cultivated germplasm. The GC–MS analysis of n-hexane extracts identified 27 volatile compounds, with citral isomers being the most prevalent. The E-citral (geranial) content ranged from 22.480 to 67.577%, while Z-citral (neral) ranged from 10.027 to 25.107%. This observation indicates significant chemotypic variation among the accessions. Notably, there was also variation in geraniol, β-myrcene, and geranyl acetate. Accessions SK, BK, V1, V3, TN1, and PY1 exhibited high levels of citral and geraniol making them strong candidates for breeding, production, and chemotype stabilization. In contrast, accessions such as V2, QN2, HCM1, QT3, and BD3 significantly higher levels of geraniol, indicating distinct geraniol-rich chemotypes within the germplasm. This study highlighted accession SK as a superior high-citral genotype, with a citral content of 86.530%. Additionally, accessions with elevated geraniol levels were identified, including PY1 at 8.567%, underscoring the valuable chemotypic diversity present in the germplasm. The integration of molecular and chemical data helped clarify the relationships between different genotypes and chemotypes. This provides a solid foundation for selecting elite germplasm with consistent essential oil quality, thereby supporting the sustainable breeding and conservation of lemongrass genetic resources in Southeast Asia.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrated molecular and phytochemical characterization of lemongrass (Cymbopogon spp.) germplasm from Vietnam and Laos

  • 期刊论文

  • 发表日期：

    2026年2月16日

查看全文
  • Hai Thi Hong Truong,
  • Nhi Thi Hoang Ho,
  • Han Ngoc Ho,
  • Thuy Thanh Duong

摘要

This study evaluated the genetic and phytochemical diversity of 35 Cymbopogon accessions from Vietnam and Laos using an integrated approach combining ITS sequencing, random amplified polymorphic DNA (RAPD) markers, and GC–MS profiling. ITS analysis identified 33 accessions as Cymbopogon citratus and two as Cymbopogon winterianus, revealing only two major haplotypes and indicating low intraspecific variation, likely due to clonal propagation. RAPD analysis using eight highly polymorphic primers generated 86 bands with 61.28% polymorphism, indicating moderate genetic diversity and forming two distinct clusters corresponding to two species boundaries. The lack of clear geographic structuring suggests extensive exchange of planting materials, resulting in the genetic homogenization of cultivated germplasm. The GC–MS analysis of n-hexane extracts identified 27 volatile compounds, with citral isomers being the most prevalent. The E-citral (geranial) content ranged from 22.480 to 67.577%, while Z-citral (neral) ranged from 10.027 to 25.107%. This observation indicates significant chemotypic variation among the accessions. Notably, there was also variation in geraniol, β-myrcene, and geranyl acetate. Accessions SK, BK, V1, V3, TN1, and PY1 exhibited high levels of citral and geraniol making them strong candidates for breeding, production, and chemotype stabilization. In contrast, accessions such as V2, QN2, HCM1, QT3, and BD3 significantly higher levels of geraniol, indicating distinct geraniol-rich chemotypes within the germplasm. This study highlighted accession SK as a superior high-citral genotype, with a citral content of 86.530%. Additionally, accessions with elevated geraniol levels were identified, including PY1 at 8.567%, underscoring the valuable chemotypic diversity present in the germplasm. The integration of molecular and chemical data helped clarify the relationships between different genotypes and chemotypes. This provides a solid foundation for selecting elite germplasm with consistent essential oil quality, thereby supporting the sustainable breeding and conservation of lemongrass genetic resources in Southeast Asia.