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<p>Actin depolymerizing factor (ADF) is a small actin-binding protein (ABP) that primarily severs and depolymerizes filamentous actin (F-actin) assembly, thereby regulating various cellular, molecular, and biological processes in plants. However, the function of ADF in <i>Brassica&#xa0;napus</i> remains unexplore. additionally, high-temperature stress during flowering significantly reduce fertility and yield, emphasizing the important of studying ADFs under these conditions. In this study, we identified 32 ADF genes, unevenly distributed across 18 chromosomes, in <i>B. napus</i> using various bioinformatic methods.&#xa0;Phylogenetic analysis revealed that all BnADFs clustered into five distinct clades, alongside <i>Arabidopsis</i> <i>thaliana</i> ADFs. Gene duplication analysis identified one tandem duplication and 75 segmental duplication events, which contributed to the expansion of ADF in <i>B. napus</i> and have been shape by purifying selection during evolution. The Cis-acting element prediction revealed numerous regulatory elements involved in hormone signaling and responses to abiotic stress. Tissue-specific expression of BnADFs showed diverse expression patterns across vegetative and reproductive tissues. The transcriptional analysis of floral buds under high-temperature stress indicated that not all BnADF genes were significantly induced. However, <i>BnADF2, BnADF7</i>, <i>BnADF9, BnADF13, BnADF16, BnADF19, BnADF20</i>, <i>BnADF27,</i> and <i>BnADF29 (</i>homologs of <i>AtADF7</i> and <i>AtADF10)</i> showed significant responses to high temperature stress. These genes are promising candidates for investigating the molecular mechanisms underlying the role of ADFs in response to HTS in <i>B. napus</i> during the flowering stage<i>.</i> The research lay the groundwork for improved agricultural practices and the development of more resilient crops.</p> Graphical Abstract <p></p>

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In-silico genome-wide identification of ADF gene family in Brassica napus under long-term high-temperature stress

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Enerand Mackon,
  • Shanshan Zhang,
  • Zhuyu Pan,
  • Muhammad Ikram,
  • Latif Ullah Khan,
  • Wajid Saeed,
  • Muhammad Waseem,
  • Yun Li,
  • Pingwu Liu

摘要

Actin depolymerizing factor (ADF) is a small actin-binding protein (ABP) that primarily severs and depolymerizes filamentous actin (F-actin) assembly, thereby regulating various cellular, molecular, and biological processes in plants. However, the function of ADF in Brassica napus remains unexplore. additionally, high-temperature stress during flowering significantly reduce fertility and yield, emphasizing the important of studying ADFs under these conditions. In this study, we identified 32 ADF genes, unevenly distributed across 18 chromosomes, in B. napus using various bioinformatic methods. Phylogenetic analysis revealed that all BnADFs clustered into five distinct clades, alongside Arabidopsis thaliana ADFs. Gene duplication analysis identified one tandem duplication and 75 segmental duplication events, which contributed to the expansion of ADF in B. napus and have been shape by purifying selection during evolution. The Cis-acting element prediction revealed numerous regulatory elements involved in hormone signaling and responses to abiotic stress. Tissue-specific expression of BnADFs showed diverse expression patterns across vegetative and reproductive tissues. The transcriptional analysis of floral buds under high-temperature stress indicated that not all BnADF genes were significantly induced. However, BnADF2, BnADF7, BnADF9, BnADF13, BnADF16, BnADF19, BnADF20, BnADF27, and BnADF29 (homologs of AtADF7 and AtADF10) showed significant responses to high temperature stress. These genes are promising candidates for investigating the molecular mechanisms underlying the role of ADFs in response to HTS in B. napus during the flowering stage. The research lay the groundwork for improved agricultural practices and the development of more resilient crops.

Graphical Abstract