Scholarlink

<p>Indonesia hosts the richest genetic diversity of <i>Musa acuminata</i> Colla in Southeast Asia. This wild banana species is a vital genetic resources for the crop improvement of edible bananas, especially those subspecies with traits conferring disease resistance. This study aimed to develop a versatile in vitro regeneration medium for six varieties of <i>M. acuminata</i> and evaluate cryopreservation methods for seeds. Plant materials were collected from existing germplasm collections of the National Research and Innovation Agency, Indonesia. The optimal regeneration medium consisted of Murashige and Skoog’s salts supplemented with 4.44–8.88&#xa0;μM benzyladenine and 0.5&#xa0;μM thidiazuron, achieving over 80% germination and supporting plantlet formation and shoot proliferation after four weeks. Air-desiccated seeds (5–14% moisture content) followed by fast freezing proved effective for var. <i>tomentosa</i> as its post-freezing germination remained high at up to 100%. The protocol could also be extended to other varieties such as var. <i>rutilifes</i>, var. <i>microcarpa</i>, var. <i>sumatrana</i>, var. <i>malaccensis</i>, and var. <i>halabanensis</i> as their post freezing germination varied from 20 to 70%. As seeds of these varieties could tolerate desiccation to 5–14% moisture content, these varieties could be classified into an intermediated seed storage behavior type. Further evaluation is needed for other infraspecific taxa of wild <i>M. acuminata</i> bananas such as subsp. <i>banksii</i>, var. <i>nakaii</i>, var. <i>zebrina</i>, var. <i>bantamensis</i>, var. <i>longipetiolata</i>, and var. <i>breviformis</i>, many of which are endemic to the Indonesian archipelago.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Regeneration medium and simple freezing improve cryopreservation of wild banana varieties (Musa acuminata Colla)

  • 期刊论文

  • 发表日期：

    2026年1月5日

查看全文
  • Apriliana Dyah Prawestri,
  • Eka Martha Della Rahayu,
  • Yuyu S. Poerba,
  • Tri Handayani,
  • Fajarudin Ahmad,
  • Yayan Wahyu Candra Kusuma,
  • La Ode Muhammad Muchdar Davis,
  • Enny Sudarmonowati,
  • Witjaksono Witjaksono,
  • Bart Panis,
  • Ika Roostika

摘要

Indonesia hosts the richest genetic diversity of Musa acuminata Colla in Southeast Asia. This wild banana species is a vital genetic resources for the crop improvement of edible bananas, especially those subspecies with traits conferring disease resistance. This study aimed to develop a versatile in vitro regeneration medium for six varieties of M. acuminata and evaluate cryopreservation methods for seeds. Plant materials were collected from existing germplasm collections of the National Research and Innovation Agency, Indonesia. The optimal regeneration medium consisted of Murashige and Skoog’s salts supplemented with 4.44–8.88 μM benzyladenine and 0.5 μM thidiazuron, achieving over 80% germination and supporting plantlet formation and shoot proliferation after four weeks. Air-desiccated seeds (5–14% moisture content) followed by fast freezing proved effective for var. tomentosa as its post-freezing germination remained high at up to 100%. The protocol could also be extended to other varieties such as var. rutilifes, var. microcarpa, var. sumatrana, var. malaccensis, and var. halabanensis as their post freezing germination varied from 20 to 70%. As seeds of these varieties could tolerate desiccation to 5–14% moisture content, these varieties could be classified into an intermediated seed storage behavior type. Further evaluation is needed for other infraspecific taxa of wild M. acuminata bananas such as subsp. banksii, var. nakaii, var. zebrina, var. bantamensis, var. longipetiolata, and var. breviformis, many of which are endemic to the Indonesian archipelago.