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<p>WD40 proteins constitute a substantial gene family within eukaryotic organisms, exhibiting a variety of biological functions. However, a comprehensive identification and analysis of WD40 proteins in carrot (<i>Daucus carota</i> L.) has not been conducted. This study identified 278 <i>WD40</i> genes in carrot and assessed their chromosomal distribution, gene structure, and evolutionary relationships. The identified <i>WD40</i> genes were found to be unevenly distributed across nine chromosomes. These genes were classified into 12 distinct subfamilies based on their domain composition. The analysis of the replication patterns of the <i>DcWD40</i> genes revealed that tandem duplication serves as the primary mode for the amplification of the <i>DcWD40</i> gene family. Ka/Ks analysis indicated that all <i>WD40</i> duplicated gene pairs have undergone purifying selection throughout their evolutionary history. RNA-seq data from various carrot tissues and qRT-PCR analysis under dark and drought stress revealed diverse expression patterns of the <i>DcWD40</i> genes. Certain genes exhibited tissue-specific expression, while the majority of genes showed consistent expression levels across various tissues and developmental stages of carrot. This study enhances our understanding of the <i>WD40</i> gene family in carrot and establishes a valuable basis for future investigations into the functional roles of <i>WD40</i> genes.</p>

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Genome-wide identification and bioinformatics analysis of WD40 gene family in carrot (Daucus carota L.)

  • 期刊论文

  • 发表日期：

    2025年9月5日

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  • Jie Wu,
  • Feijuan Gao,
  • Hao Sun,
  • Xiaoping Kong,
  • Xiangping Yan,
  • Hongwei Zhou,
  • Liwen Chen

摘要

WD40 proteins constitute a substantial gene family within eukaryotic organisms, exhibiting a variety of biological functions. However, a comprehensive identification and analysis of WD40 proteins in carrot (Daucus carota L.) has not been conducted. This study identified 278 WD40 genes in carrot and assessed their chromosomal distribution, gene structure, and evolutionary relationships. The identified WD40 genes were found to be unevenly distributed across nine chromosomes. These genes were classified into 12 distinct subfamilies based on their domain composition. The analysis of the replication patterns of the DcWD40 genes revealed that tandem duplication serves as the primary mode for the amplification of the DcWD40 gene family. Ka/Ks analysis indicated that all WD40 duplicated gene pairs have undergone purifying selection throughout their evolutionary history. RNA-seq data from various carrot tissues and qRT-PCR analysis under dark and drought stress revealed diverse expression patterns of the DcWD40 genes. Certain genes exhibited tissue-specific expression, while the majority of genes showed consistent expression levels across various tissues and developmental stages of carrot. This study enhances our understanding of the WD40 gene family in carrot and establishes a valuable basis for future investigations into the functional roles of WD40 genes.