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<p>Tuberose (<i>Agave amica</i>), a commercially significant ornamental plant, exhibits limited genetic diversity due to restricted breeding efforts. Assessing genetic variation is essential for breeding advancements and conservation strategies. This study aimed to evaluate the agro-morphological differentiation and genetic diversity among 13 tuberose cultivars using multivariate phenotypic analysis and sequence-related amplified polymorphism (SRAP) markers. Multifactorial Analysis (MFA) identified four principal factors, explaining 84.103% of the total variation, with plant height, spike length, and flower length being major contributors. Agglomerative Hierarchical Clustering (AHC) further categorized genotypes based on floral traits and genetic similarity. The 80 SRAP primer combinations were tested, 14 produced consistent and scorable amplification patterns, generating 63 bands, of which 54 (81%) were polymorphic. The polymorphic information content (PIC) ranged from 0.07 to 0.92, with an average of 0.52, indicating high level of marker informativeness. Jaccard’s similarity coefficients ranged from 0.51 to 0.89, reflecting significant genetic variability. &#xa0;Unweighted pair-group method with arithmetic mean (UPGMA) clustering grouped the cultivars into two major clusters, aligning their genetic background and morphological traits. Comparative analysis of molecular and morphological clustering revealed both congruence and discrepancies, emphasizing the influence of genetic background and environmental factors on phenotypic expression. This study provides a robust framework for marker-assisted selection and conservation strategies, ensuring the genetic sustainability and improvement of tuberose cultivars.</p>

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Unravelling genetic and phenotypic variability in Agave amica across India using sequence-related amplified polymorphism (SRAP) markers

  • 期刊论文

  • 发表日期：

    2025年6月13日

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  • P. R. Jadhav,
  • A. Y. Jagtap,
  • P. R. Shingote,
  • A. U. Solanke,
  • M. C. Pagariya,
  • P. N. Kumar,
  • K. V. Prasad,
  • P. G. Kawar

摘要

Tuberose (Agave amica), a commercially significant ornamental plant, exhibits limited genetic diversity due to restricted breeding efforts. Assessing genetic variation is essential for breeding advancements and conservation strategies. This study aimed to evaluate the agro-morphological differentiation and genetic diversity among 13 tuberose cultivars using multivariate phenotypic analysis and sequence-related amplified polymorphism (SRAP) markers. Multifactorial Analysis (MFA) identified four principal factors, explaining 84.103% of the total variation, with plant height, spike length, and flower length being major contributors. Agglomerative Hierarchical Clustering (AHC) further categorized genotypes based on floral traits and genetic similarity. The 80 SRAP primer combinations were tested, 14 produced consistent and scorable amplification patterns, generating 63 bands, of which 54 (81%) were polymorphic. The polymorphic information content (PIC) ranged from 0.07 to 0.92, with an average of 0.52, indicating high level of marker informativeness. Jaccard’s similarity coefficients ranged from 0.51 to 0.89, reflecting significant genetic variability.  Unweighted pair-group method with arithmetic mean (UPGMA) clustering grouped the cultivars into two major clusters, aligning their genetic background and morphological traits. Comparative analysis of molecular and morphological clustering revealed both congruence and discrepancies, emphasizing the influence of genetic background and environmental factors on phenotypic expression. This study provides a robust framework for marker-assisted selection and conservation strategies, ensuring the genetic sustainability and improvement of tuberose cultivars.