<p>Kodo millet (<i>Paspalum scrobiculatum</i> L.) is one of the minor millets, valued for its rich nutritional profile and is commonly consumed in India. Although many investigations have focused on grain yield and associated traits, and biotic and abiotic stress tolerance in this millet, there are limited reports on recombination breeding efforts as it is constrained by lack of effective emasculation and crossing technique owing to the small and delicate spikelets, unusual anthesis behaviour, etc. Therefore, a study was carried out to examine floral biology, flowering behaviour and spikelet arrangement pattern in a set of germplasm accessions. Out of 144 accessions planted in the field in a replicated trial, 82% had regular arrangement of spikelets on the rachis, 14% showed irregular arrangement, and rest 4% displayed combination of both types on the same rachis. Spikelet opening could be observed in the open field as early as 6.00 am in the morning (average temperature of 16− 18&#xa0;^°C and RH of 70–80%) with sequence of events like opening of glumes, stigma and anther exsertion, anther dehiscence, retraction of stigma and closing of glumes. The detailed account of floral biology and flowering behaviour reported here can assist in attempting artificial hybridization by identifying the appropriate raceme where flowering has just begun and carrying out hand emasculation and crossing. This would greatly help the kodo millet breeders in varietal development through recombination breeding, which is highly essential for developing variability for complex traits such as grain yield, abiotic and biotic stress tolerance as well as nutritional quality.</p>