<p>Genetic resources play a crucial role in plant evolution, particularly in the context of developing cultivars that can withstand environmental stress. Identifying and conserving salt-tolerant species is crucial for crop resilience and food security in saline-affected areas. Therefore, pollen morphological study of 12 halophytes (<i>Leptadenia pyrotechnica, Oxystelma esculentum, Leucaena leucocephala, Neltuma juliflora, Prosopis cineraria, Vachellia nilotica, Oligomeris linifolia, Withania coagulans, Solanum incanum, Withania somnifera, Tamarix aphylla,</i> and <i>Tamarix dioica</i>) from Salt range of North Punjab, Pakistan using light microscopy (LM) and scanning electron microscopy (SEM) is presented in current study. The main objective was to describe the significance of pollen morphology, which is useful for accurately identifying and delineating plants based on habitat. Different examined pollen features such as aperture (tricolporate, tricolpate, trizonocolporate), shape (oblate-spheroidal, suboblate, prolate-spheroidal), and exine sculpture (reticulate, rugulate, fossulate, foveolate, scabrate) are beneficial to support and strengthen the systematics of halophytes. All species exhibited high pollen viability values (%) that confirm their environmental adaptability in the Salt ranges. Cluster and Principal component analysis (PCA) were performed to show correlations between the species. The calculated correlation for the pollen traits indicated a positive correlation between the polar and equatorial diameters, exhibiting shape similarity in most halophytes. Quantitative data along with qualitative traits assist in the species identification and delimitation among the same group taxa. Pollen morphological characters such as polar and equatorial axis, exine sculpture, and aperture provide a useful reference for the systematic classification of halophytes.</p> Graphical abstract <p></p>