Scholarlink

<p>The genus <i>Chenopodium</i> which includes <i>C. album</i> and <i>C. quinoa,</i> is an essential under-utilized pseudo-cereal crop. Owing to its potent nutraceutical benefits, the cultivation of <i>Chenopodium</i> has been increasing worldwide. However, morphological heterogeneity create hindrance in the identification and differentiation within&#xa0;or&#xa0;between accessions of these two species due to the significant variability in traits such as plant height, leaf shape, and seed morphology. The present study is an attempt to assess the diversity in morphological traits, cytogenetic characteristics (ploidy number and karyotyping) and determine the variation of total nuclear DNA content between 50 distinct, <i>C. album</i> and <i>C. quinoa</i> accessions. Flow cytometry analysis from our study stated a high level of consistency within the same accession in the biological replicates. The average value of 2C DNA for <i>C. quinoa</i> (3.38 ± 0.07&#xa0;pg) was significantly different from the <i>C. album</i> (3.85 ± 0.06&#xa0;pg). Karyotypic analysis showed <i>C. quinoa</i> to have ploidy level 2n = 4x = 36, while in <i>C. album</i> both 2n = 4x = 36 and 2n = 6x = 54, x = 9 ploidy levels. Within the complements of various accessions, <i>C. album</i> (4 × and 6x) and <i>C. quinoa</i> (4x) exhibit minor yet consistent variation in arm ratio of different chromosomes. The chromosomes arrangement of <i>C. quinoa</i>, with 18 pairs which confirmed the species’ as allotetraploidy whereas&#xa0;<i>C. album</i>&#xa0;complex showed 4 × and 6 × cytotypes; arranged in 18 and 27 pairs suggest tetraploidy and hexaploidy nature, respectively. In contrast to the 6 × cytotype, the 4 × cytotype exhibits a more asymmetrical karyotype. The findings of this study demonstrate that <i>Chenopodium spp</i>. possess highly stable genomic content, which further supports the paraphyletic origin of the genus <i>Chenopodium</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Diversity assessment of Chenopodium album and Chenopodium quinoa based on the morphometric traits, chromosome analysis and total nuclear DNA content

  • 期刊论文

  • 发表日期：

    2025年5月3日

查看全文
  • Babita Kumari,
  • Akib Ali,
  • Nikhil Kumar Chrungoo

摘要

The genus Chenopodium which includes C. album and C. quinoa, is an essential under-utilized pseudo-cereal crop. Owing to its potent nutraceutical benefits, the cultivation of Chenopodium has been increasing worldwide. However, morphological heterogeneity create hindrance in the identification and differentiation within or between accessions of these two species due to the significant variability in traits such as plant height, leaf shape, and seed morphology. The present study is an attempt to assess the diversity in morphological traits, cytogenetic characteristics (ploidy number and karyotyping) and determine the variation of total nuclear DNA content between 50 distinct, C. album and C. quinoa accessions. Flow cytometry analysis from our study stated a high level of consistency within the same accession in the biological replicates. The average value of 2C DNA for C. quinoa (3.38 ± 0.07 pg) was significantly different from the C. album (3.85 ± 0.06 pg). Karyotypic analysis showed C. quinoa to have ploidy level 2n = 4x = 36, while in C. album both 2n = 4x = 36 and 2n = 6x = 54, x = 9 ploidy levels. Within the complements of various accessions, C. album (4 × and 6x) and C. quinoa (4x) exhibit minor yet consistent variation in arm ratio of different chromosomes. The chromosomes arrangement of C. quinoa, with 18 pairs which confirmed the species’ as allotetraploidy whereas C. album complex showed 4 × and 6 × cytotypes; arranged in 18 and 27 pairs suggest tetraploidy and hexaploidy nature, respectively. In contrast to the 6 × cytotype, the 4 × cytotype exhibits a more asymmetrical karyotype. The findings of this study demonstrate that Chenopodium spp. possess highly stable genomic content, which further supports the paraphyletic origin of the genus Chenopodium.