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<p><i>Kengyilia grandiglumis</i> (Keng) J. L. Yang et al. is a perennial grass that developed in the sandy habitat of the Qinghai-Tibet Plateau. In this study, three-color FISH combined with sequential GISH was carried out on 80 individuals from 4 representative populations of <i>K. grandiglumis</i>. For the first time, two types of reciprocal translocations between the <b>P</b> and <b>Y</b> subgenomes have been identified in <i>K. grandiglumis</i>. These translocations were observed in populations QH1 and QH2, respectively, and the translocation chromosomes and frequency were different. The signals of the tandem repeat probe HvT01 were represented on chromosomes 3P, 6P, 7P, 1St, 2St, 5St, and 3Y, most of which were located on the subtelomeric regions. The simple sequence repeat AAG tended to be distributed on the pericentromeric regions and was detected on chromosomes 1 to 7Y, 2St, 3St, 4St, 5St, 6St and 6P. The chromosomal distribution of the TE probe S5 was concentrated on the telomere to intercalary regions of chromosomes 1P-7P, whereas in the <b>Y</b> and <b>St</b> subgenomes, the signal was scattered on the subtelomeric or pericentromeric regions of some chromosomes. On the basis of the diversity of the three probe signals within and among the 4 populations, we mapped the idiogram of FISH-banded chromosomes of <i>K. grandiglumis</i>. These results provide important theoretical guidance for the collection, protection, and evaluation of <i>K. grandiglumis</i>.</p>

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Analysis of the molecular karyotype of Kengyilia grandiglumis on the Qinghai-Tibet Plateau via sequential FISH and GISH

  • 期刊论文

  • 发表日期：

    2025年3月19日

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  • Ruijuan Liu,
  • Liling Jiang,
  • Yijun Zhong,
  • Yue Wu,
  • Demei Liu,
  • Wenjie Chen

摘要

Kengyilia grandiglumis (Keng) J. L. Yang et al. is a perennial grass that developed in the sandy habitat of the Qinghai-Tibet Plateau. In this study, three-color FISH combined with sequential GISH was carried out on 80 individuals from 4 representative populations of K. grandiglumis. For the first time, two types of reciprocal translocations between the P and Y subgenomes have been identified in K. grandiglumis. These translocations were observed in populations QH1 and QH2, respectively, and the translocation chromosomes and frequency were different. The signals of the tandem repeat probe HvT01 were represented on chromosomes 3P, 6P, 7P, 1St, 2St, 5St, and 3Y, most of which were located on the subtelomeric regions. The simple sequence repeat AAG tended to be distributed on the pericentromeric regions and was detected on chromosomes 1 to 7Y, 2St, 3St, 4St, 5St, 6St and 6P. The chromosomal distribution of the TE probe S5 was concentrated on the telomere to intercalary regions of chromosomes 1P-7P, whereas in the Y and St subgenomes, the signal was scattered on the subtelomeric or pericentromeric regions of some chromosomes. On the basis of the diversity of the three probe signals within and among the 4 populations, we mapped the idiogram of FISH-banded chromosomes of K. grandiglumis. These results provide important theoretical guidance for the collection, protection, and evaluation of K. grandiglumis.