<p>Although crop leaf traits associated with acquisitive life strategies might largely be the result of inherence from their wild progenitors, more research is still needed to understand the roles that genotype and environment play in their expression. Given their importance for regulating photosynthesis and protecting plants from herbivores, it is surprising that only a handful of investigations have systematically explored how crop selection history influenced leaf evolution. Hence, we examined the direct and interactive effects of crop domestication and nitrogen (N) fertilization on leaf functional traits (leaf area, specific leaf area, relative chlorophyll content, and trichome density at leaf edges) across three Mexican crops. Overall, leaf traits were directly and interactively influenced by both genotype and environment. Specifically, we found that multiple acquisitive traits on domesticated phenotypes are either the direct plastic response to soil N availability or the result of an interaction between crop domestication and mineral fertilization (rather than solely arising as an evolutionary consequence of domestication itself). Moreover, it appears that&#xa0;crop domestication has impacted the capacity of plants to respond to N fertilization, likely due to alterations in phenotypic plasticity. Thus, our results will not only contribute to the growing literature of crop evolution, but demonstrate how domesticated plants may have adapted to agroecosystems via fundamental genotype-environment interactions.</p>