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<p>‘Red-fleshed pomelo’ (<i>Citrus maxima</i>) is a spontaneous bud mutant from ‘Guanxi pummelo’ that accumulates abundant lycopene and β-carotene in the juice sacs of mature fruits, however, the regulatory mechanism of lycopene and β-carotene accumulation in ‘Red-fleshed pomelo’ fruits remains to be elucidated. In this study, a <i>RING finger</i> gene <i>CmXERICO</i> and its promoter were isolated from the carotenoid-accumulating mutant ‘Red-fleshed pomelo’, and characterized through virus-induced gene silencing (VIGS) and stable heterologous overexpression. Sequence analysis revealed that CmXERICO contained an N-terminal transmembrane domain and a highly conserved C3H2C3-type RING finger domain at the C-terminus. The <i>CmXERICO</i> gene was constitutively expressed in different vegetative and reproductive organs, and was expressed at higher levels in the juice sacs of mature ‘Red-fleshed pomelo’ fruits than those of wild-type ‘Guanxi pomelo’ fruits. Transient silencing of <i>CmXERICO</i> in mature-green tomatoes significantly decreased the total carotenoid content by reducing the accumulation of lycopene and β-carotene. The <i>CmXERICO</i> promoter contains diverse regulatory elements related to MYB, MYC, ethylene, salicylic acid and light responses. Additionally, <i>CmXERICO</i> promoter strongly drove β-glucuronidase (GUS) activity during the early plant growth and development. Taken together, these results indicate that <i>CmXERICO</i> positively affects the accumulation of lycopene and β-carotene in fruit, and is involved in regulating plant morphogenesis.</p>

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Characterization of CmXERICO gene regulating carotenoid accumulation and morphogenesis in Citrus maxima

  • 期刊论文

  • 发表日期：

    2025年3月15日

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  • Haoran Zhang,
  • Jiaxing Zheng,
  • Nan Bai,
  • Xiaoqin Jin,
  • Wenbo Lin,
  • Changchun Wang

摘要

‘Red-fleshed pomelo’ (Citrus maxima) is a spontaneous bud mutant from ‘Guanxi pummelo’ that accumulates abundant lycopene and β-carotene in the juice sacs of mature fruits, however, the regulatory mechanism of lycopene and β-carotene accumulation in ‘Red-fleshed pomelo’ fruits remains to be elucidated. In this study, a RING finger gene CmXERICO and its promoter were isolated from the carotenoid-accumulating mutant ‘Red-fleshed pomelo’, and characterized through virus-induced gene silencing (VIGS) and stable heterologous overexpression. Sequence analysis revealed that CmXERICO contained an N-terminal transmembrane domain and a highly conserved C3H2C3-type RING finger domain at the C-terminus. The CmXERICO gene was constitutively expressed in different vegetative and reproductive organs, and was expressed at higher levels in the juice sacs of mature ‘Red-fleshed pomelo’ fruits than those of wild-type ‘Guanxi pomelo’ fruits. Transient silencing of CmXERICO in mature-green tomatoes significantly decreased the total carotenoid content by reducing the accumulation of lycopene and β-carotene. The CmXERICO promoter contains diverse regulatory elements related to MYB, MYC, ethylene, salicylic acid and light responses. Additionally, CmXERICO promoter strongly drove β-glucuronidase (GUS) activity during the early plant growth and development. Taken together, these results indicate that CmXERICO positively affects the accumulation of lycopene and β-carotene in fruit, and is involved in regulating plant morphogenesis.