Diversity in protein, lipids, and γ-oryzanol contents of glutinous rice landraces in northwest Vietnam
摘要
Glutinous rice contains a lot of beneficial phytonutrients such as protein, lipid, and oryzanol. However, genetic data of these components are limited in landraces in Northwest Vietnam to use these genetic resources. This study was conducted to investigate varietal differences of the protein, lipid, and oryzanol content and analyze the genetic diversity using 35 simple sequence repeat (SSR) markers in 25 sticky rice landraces over three years. The obtained results showed that 25 out of the 35 SSR primers showed distinct, reproducible polymorphism. A dendrogram constructed using the SSR primers clustered the 25 glutinous rice accessions into five groups. Most of the quality traits in rice differed among the genotypes but not by year or interaction year and genotypes. Lipids in rice grain ranged from 2.64 to 2.98%, while that in bran showed a variation from 18.8 to 22.7%, respectively. Protein and γ-oryzanol content in the grain was from 7.56 to 12.22% and from 50.1 to 66.7 mg 100 g−1, respectively. Among tested accessions, 8-TNSC accession was the superior genotype for protein content with 12.22%. Three accessions, viz., 24-TDSC, 25-TLDB, and 18-TPDLC showed high lipid content in the bran. Thirteen of 25 genotypes showed γ-oryzanol contents higher than 60 mg 100 g−1, particularly, 5-TLSC and 25-TLDB showed the highest value of γ-oryzanol contents with 66.7 and 66.2 mg 100 g−1, respectively. The superior accessions would be used as potential donors for improving the lipid, protein, and γ-oryzanol content through rice breeding.