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<p><i>Pinus</i> species are distributed over a wide area of DPR Korea and plays an important role in the context of ecological conservation and economic use. To contribute to the establishment of conservation strategies for <i>Pinus</i> species, DNA barcodes and Sequence Related Amplified Polymorphism (SRAP) markers were used to reveal genetic diversity and relationships among five species and one mutant belonging to genus <i>Pinus</i>. The dendrogram based on <i>ITS</i> + <i>trnT-trnL</i> divided all samples into four clusters at genetic distance 0.002; <i>Pinus densiflora</i> + <i>Pinus densiflora</i> Form octopartina + <i>Pinus thunbergii</i>, <i>Pinus silvestris</i>, <i>Pinus tabuliformis</i> and <i>Pinus rigida</i>. On the other hand, for <i>Pinus</i> species used in this study, 169 bands obtained from 10 SRAP primer pairs with proportion of polymorphic bands was 78.7%, Nei’s diversity index was 0.0591–0.1028, and Shannon information index was 0.1002–0.1498. The analysis of molecular variance (AMOVA) revealed that 67.89% of the total variance was due to differences between species. SRAP marker-based unweighted pair group method with arithmetic mean (UPGMA) dendrogram divided all samples into five clusters as revealed by DNA barcode at genetic distance of 0.45, and the cluster <i>P. densiflora</i> + <i>P. densiflora</i> Form <i>octopartina</i> Mayr was divided into <i>P. densiflora</i> and <i>P. densiflora</i> Form <i>octopartina</i> at genetic distance of 0.12. These results suggest efficiency of DNA barcodes and SRAP markers in molecular classification and genetic analysis of <i>Pinus</i> species and will aid to establish afforestation and conservation strategies for <i>Pinus</i> species.</p>

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DNA barcode and sequence related amplified polymorphism markers reveal phylogenetic relationship among Pinus species representing closed lineage divergence

  • 期刊论文

  • 发表日期：

    2025年1月21日

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  • Mi-Ryong Choe,
  • Sam-Rang Song,
  • Un–Hyang Ho,
  • Jong–Gum Ri,
  • Chung-Il Yo,
  • Tong-Ryul Kim,
  • Ok-Hyang Han,
  • Il-Jin Kim,
  • Chol-Ho Kang

摘要

Pinus species are distributed over a wide area of DPR Korea and plays an important role in the context of ecological conservation and economic use. To contribute to the establishment of conservation strategies for Pinus species, DNA barcodes and Sequence Related Amplified Polymorphism (SRAP) markers were used to reveal genetic diversity and relationships among five species and one mutant belonging to genus Pinus. The dendrogram based on ITS + trnT-trnL divided all samples into four clusters at genetic distance 0.002; Pinus densiflora + Pinus densiflora Form octopartina + Pinus thunbergii, Pinus silvestris, Pinus tabuliformis and Pinus rigida. On the other hand, for Pinus species used in this study, 169 bands obtained from 10 SRAP primer pairs with proportion of polymorphic bands was 78.7%, Nei’s diversity index was 0.0591–0.1028, and Shannon information index was 0.1002–0.1498. The analysis of molecular variance (AMOVA) revealed that 67.89% of the total variance was due to differences between species. SRAP marker-based unweighted pair group method with arithmetic mean (UPGMA) dendrogram divided all samples into five clusters as revealed by DNA barcode at genetic distance of 0.45, and the cluster P. densiflora + P. densiflora Form octopartina Mayr was divided into P. densiflora and P. densiflora Form octopartina at genetic distance of 0.12. These results suggest efficiency of DNA barcodes and SRAP markers in molecular classification and genetic analysis of Pinus species and will aid to establish afforestation and conservation strategies for Pinus species.