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<p>The experimental laboratory studies were carried out on fresh-water Prussian carp (<i>Carassius auratus gibelio</i> Bloch, 1782) and common carp (<i>Cyprinus carpio</i>) to determine the role of the collapsin-response mediator protein 2 (CRMP2) in the adaptation of these freshwater fish to a brackish water environment. Behavioral experiments were carried out on a water maze with one of the compartments filled with brackish water (BWC), and the other one with fresh water (FWC). The levels of CRMP2 and heat shock protein with <i>M</i><sub><i>r</i></sub> 70&#xa0;kDa in the tissues were defined by indirect ELISA test. Keeping the experimental group Prussian carp specimens in water containing 5‰ NaCl for 48&#xa0;h brought to their preferential choice of the BWC. Conversely, blocking the activity of CRMP2 through intra-cerebral administration of anti-CRMP2 antibody to salinity-preconditioned fish induced their preferential choice of the FWC, whereas in the positive control group administration of non-immune γ-globulins did not change the salinity-preconditioned preference to the BWC. Indirect ELISA test analysis of the brain and liver of the salinity-preconditioned specimens revealed CRMP2 upregulation in their brains. Intra-cerebral administration of CRMP2 to the common carp specimens resulted in preferential choice of the BWC relative to the controls (administration of heat-inactivated CRMP2) and intact specimens. Intra-cerebral administration of CRMP2 to the common carp specimens brought 4&#xa0;h later to HSP70 upregulation in their muscles and livers. The results indicate prominent role for the brain CRMP2 in animals’ adaptation to adverse environmental conditions and that this adaptation may be realized by upregulation of HSP70 in the peripheral tissues.</p>

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Studies for the role of CRMP2 of brain cells in adaptation of fresh water fish to increased salinity and for underlying mechanisms

  • 期刊论文

  • 发表日期：

    2026年7月1日

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  • Arif A. Mekhtiev,
  • Namig J. Mustafayev,
  • Gunay G. Rahimova,
  • Gulshan B. Mustafayeva

摘要

The experimental laboratory studies were carried out on fresh-water Prussian carp (Carassius auratus gibelio Bloch, 1782) and common carp (Cyprinus carpio) to determine the role of the collapsin-response mediator protein 2 (CRMP2) in the adaptation of these freshwater fish to a brackish water environment. Behavioral experiments were carried out on a water maze with one of the compartments filled with brackish water (BWC), and the other one with fresh water (FWC). The levels of CRMP2 and heat shock protein with Mr 70 kDa in the tissues were defined by indirect ELISA test. Keeping the experimental group Prussian carp specimens in water containing 5‰ NaCl for 48 h brought to their preferential choice of the BWC. Conversely, blocking the activity of CRMP2 through intra-cerebral administration of anti-CRMP2 antibody to salinity-preconditioned fish induced their preferential choice of the FWC, whereas in the positive control group administration of non-immune γ-globulins did not change the salinity-preconditioned preference to the BWC. Indirect ELISA test analysis of the brain and liver of the salinity-preconditioned specimens revealed CRMP2 upregulation in their brains. Intra-cerebral administration of CRMP2 to the common carp specimens resulted in preferential choice of the BWC relative to the controls (administration of heat-inactivated CRMP2) and intact specimens. Intra-cerebral administration of CRMP2 to the common carp specimens brought 4 h later to HSP70 upregulation in their muscles and livers. The results indicate prominent role for the brain CRMP2 in animals’ adaptation to adverse environmental conditions and that this adaptation may be realized by upregulation of HSP70 in the peripheral tissues.