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<p>Rainbow trout(<i>Oncorhynchus mykiss</i>) is a typical cold-water fish often threatened by high summer temperatures. Nano-selenium as a feed additive can improve the antioxidant capacity of the body and relieve stress. In this study, different levels of nano-selenium (0, 5 and 10&#xa0;mg/kg) were added to the feed of rainbow trout to determine the changes in spleen structure and expression of related genes in rainbow trout at the proper temperature (18℃) and heat stress temperature (24℃). The results showed that the addition of 5&#xa0;mg/kg nano-selenium had a more obvious protective effect on rainbow trout spleen. The addition of nano-selenium improved the antioxidant capacity and immunity of rainbow trout spleen at 18&#xa0;°C. After heat stress, the addition of 5&#xa0;mg/kg of nano-selenium significantly increased the expression levels of spleen heat shock protein genes, such as <i>HSP10, HSP47, HSP70, HSP90</i>, antioxidant-related genes, such as glutathione peroxidase (<i>GPx</i>), thioredoxin reductase (<i>Trx</i>)<i>,</i> superoxide dismutase (<i>SOD1</i>)<i>,</i> catalase (<i>CAT</i>) and immune-related genes, such as tumor necrosis factor-α (<i>TNF-α</i>), Interleukin-1β (<i>IL-1β</i>), Immunoglobulin M (<i>IgM</i>), Complement Component 3 (<i>C3</i>), resulting in the improvement of antioxidant capacity and immunity, and the protection of the spleen to alleviate the damages caused by heat stress. This study provides a theoretical basis for the development of anti-heat stress feeds for rainbow trout.</p>

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Effects of nano-selenium on the histopathological changes and gene expression of rainbow trout (Oncorhynchus mykiss) spleen under heat stress

  • 期刊论文

  • 发表日期：

    2025年9月4日

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  • Junhao Lu,
  • Zhe Liu,
  • Jiahui Zhang,
  • Lanlan Li,
  • Guiyan Zhao,
  • Yucai Pan,
  • Jinqiang Quan,
  • Guolin Song

摘要

Rainbow trout(Oncorhynchus mykiss) is a typical cold-water fish often threatened by high summer temperatures. Nano-selenium as a feed additive can improve the antioxidant capacity of the body and relieve stress. In this study, different levels of nano-selenium (0, 5 and 10 mg/kg) were added to the feed of rainbow trout to determine the changes in spleen structure and expression of related genes in rainbow trout at the proper temperature (18℃) and heat stress temperature (24℃). The results showed that the addition of 5 mg/kg nano-selenium had a more obvious protective effect on rainbow trout spleen. The addition of nano-selenium improved the antioxidant capacity and immunity of rainbow trout spleen at 18 °C. After heat stress, the addition of 5 mg/kg of nano-selenium significantly increased the expression levels of spleen heat shock protein genes, such as HSP10, HSP47, HSP70, HSP90, antioxidant-related genes, such as glutathione peroxidase (GPx), thioredoxin reductase (Trx), superoxide dismutase (SOD1), catalase (CAT) and immune-related genes, such as tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Immunoglobulin M (IgM), Complement Component 3 (C3), resulting in the improvement of antioxidant capacity and immunity, and the protection of the spleen to alleviate the damages caused by heat stress. This study provides a theoretical basis for the development of anti-heat stress feeds for rainbow trout.