<p>Flower formation is important for plant development and analyzing early-flowering genes is crucial for accelerating plant reproduction. Exploring the early fruiting genes present in <i>Vitis vinifera</i> (<i>V. vinifera</i>) can provide a molecular theoretical basis for breeding high-quality resistant varieties using the excellent quality of <i>V. vinifera</i>. In this study, the effects of a <i>V. vinifera</i> intraspecific hybrid population on the flowering characteristics of one-year-old seedlings were studied, and samples of flowering and nonflowering plants in the <i>Ecolly</i> × <i>Dornfelder</i> population were screened. High-throughput sequencing was subsequently used to perform transcriptome sequencing and related differential gene expression analysis for each sample. The results revealed that spiral vine trellising showed potential for accelerating flowering in grapevine seedlings, and 93 genes (77 upregulated and 16 downregulated) were differentially expressed between the flowering and the nonflowering groups. GO (Gene Ontology) analysis of the differentially expressed genes revealed that the pathways associated with the flowering and nonflowering groups were enriched mainly in polysaccharide binding, DNA integration, defense reactions, carbohydrate binding, and dioxygenase activity. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis of the differentially expressed genes revealed pathways that were enriched mainly in protein processing, amino sugar and nucleotide sugar metabolism, glycolysis/gluconeogenesis, pentose and glucuronic acid conversion, and pyruvate metabolism in the endoplasmic reticulum. An analysis of flowering related genes involved in various pathways revealed that the flowering genes of <i>V. vinifera</i> were related mainly to carbohydrate metabolism, secondary metabolite metabolism, amino acid metabolism, and transcription factor regulation.</p>