Scholarlink

<p>Bacterial wilt as well as shoot and fruit borer are major biotic constraints in eggplant cultivation. Developing host plant resistance is an effective method to mitigate these stresses. In this study, 30 three-way cross hybrids were generated by crossing ten half-diallel hybrids with five parental lines. The three-way cross hybrids were evaluated for bacterial wilt resistance through artificial inoculation of seedlings followed by screening in a sick plot. The hybrids were simultaneously screened for resistance to natural infestation of shoot and fruit borer. Significant variation was observed among the hybrids for per cent disease incidence (PDI), per cent shoot infestation (PSI), per cent fruit infestation (PFI) and yield components. Among three way cross hybrids, (Ponni × Vengeri) × IC636521 showed combined resistance with lowest PDI and PFI values. Based on a multi-trait selection index, 21 transgressive segregants showed superior resistance and marketable yield. Combining ability analysis revealed that non-additive gene effects predominated for yield component traits, with significant two-line (d<sub>ij</sub>, s<sub>ij</sub>) and three-line (t<sub>ijk</sub>) specific effects. Molecular validation identified the marker SSR46 as linked with bacterial wilt resistance and 5&#xa0;kb flanking region detected putative gene i.e. <i>UDP-Glucuronate: Xylanalpha-Glucuronosyl transferase</i> gene. Sequence analysis of the reading frames from resistant and susceptible genotypes revealed multiple insertions and deletions in the susceptible genotype. The resistant allele was predicted to encode a full-length 422 amino acid protein, whereas the susceptible genotype produced a truncated 348 amino acid protein. These promising transgressive segregants can be advanced to subsequent generations, and the identified marker SSR46 provides a reliable tool for marker-assisted selection (MAS) of bacterial wilt-resistant lines in eggplant breeding programs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Introgression of resistance to bacterial wilt and shoot and fruit borer in eggplant using three-way cross and insights into molecular marker functional validation

  • 期刊论文

  • 发表日期：

    2026年4月20日

查看全文
  • Kasireddy Sivasankarreddy,
  • Jiji Joseph,
  • Deepu Mathew,
  • P. P. Thirumalaisamy,
  • Berin Pathrose

摘要

Bacterial wilt as well as shoot and fruit borer are major biotic constraints in eggplant cultivation. Developing host plant resistance is an effective method to mitigate these stresses. In this study, 30 three-way cross hybrids were generated by crossing ten half-diallel hybrids with five parental lines. The three-way cross hybrids were evaluated for bacterial wilt resistance through artificial inoculation of seedlings followed by screening in a sick plot. The hybrids were simultaneously screened for resistance to natural infestation of shoot and fruit borer. Significant variation was observed among the hybrids for per cent disease incidence (PDI), per cent shoot infestation (PSI), per cent fruit infestation (PFI) and yield components. Among three way cross hybrids, (Ponni × Vengeri) × IC636521 showed combined resistance with lowest PDI and PFI values. Based on a multi-trait selection index, 21 transgressive segregants showed superior resistance and marketable yield. Combining ability analysis revealed that non-additive gene effects predominated for yield component traits, with significant two-line (dij, sij) and three-line (tijk) specific effects. Molecular validation identified the marker SSR46 as linked with bacterial wilt resistance and 5 kb flanking region detected putative gene i.e. UDP-Glucuronate: Xylanalpha-Glucuronosyl transferase gene. Sequence analysis of the reading frames from resistant and susceptible genotypes revealed multiple insertions and deletions in the susceptible genotype. The resistant allele was predicted to encode a full-length 422 amino acid protein, whereas the susceptible genotype produced a truncated 348 amino acid protein. These promising transgressive segregants can be advanced to subsequent generations, and the identified marker SSR46 provides a reliable tool for marker-assisted selection (MAS) of bacterial wilt-resistant lines in eggplant breeding programs.