<p>The present study investigated the functionality of embryo sacs in two contexts: (i) field conditions with two pollination treatments (open and cross) to observe potential differences in development and (ii) high constant temperatures within the climate change framework to examine their impact. Embryo sac functionality was evaluated in four autochthonous sweet and sour cherry genotypes over two years. In the field, flowers were collected at five-time points starting from anthesis, then embedded in paraffin, sectioned, and stained. In contrast, three-time points were utilized to assess the effects of constant temperature. The resulting histological sections were observed under a microscope. The later-flowering sweet cherry genotypes at anthesis displayed more advanced embryo sac stages. The stages of embryo sacs in sour cherry genotypes showed no consistent pattern concerning flowering time. A higher percentage of early and later stages of embryos with cellular endosperm were observed across all cherry genotypes in open compared to cross-pollination. Under constant temperatures, late-flowering sweet cherry genotypes experienced a rapid decline in embryo sac viability at both temperatures. However, this trend was not noted in sour cherry genotypes. Our histological data from autochthonous sweet and sour cherry genotypes and other pomological studies will support future breeding efforts with these genotypes.</p>

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Embryo sac functionality in autochthonous cherry genotypes grown in the Balkan region

  • Milena Đorđević,
  • Radosav Cerović,
  • Ivana Glišić,
  • Nebojša Milošević,
  • Slađana Marić,
  • Slavica Čolić,
  • Vera Rakonjac,
  • Melpomena Popovska,
  • Sanja Radičević

摘要

The present study investigated the functionality of embryo sacs in two contexts: (i) field conditions with two pollination treatments (open and cross) to observe potential differences in development and (ii) high constant temperatures within the climate change framework to examine their impact. Embryo sac functionality was evaluated in four autochthonous sweet and sour cherry genotypes over two years. In the field, flowers were collected at five-time points starting from anthesis, then embedded in paraffin, sectioned, and stained. In contrast, three-time points were utilized to assess the effects of constant temperature. The resulting histological sections were observed under a microscope. The later-flowering sweet cherry genotypes at anthesis displayed more advanced embryo sac stages. The stages of embryo sacs in sour cherry genotypes showed no consistent pattern concerning flowering time. A higher percentage of early and later stages of embryos with cellular endosperm were observed across all cherry genotypes in open compared to cross-pollination. Under constant temperatures, late-flowering sweet cherry genotypes experienced a rapid decline in embryo sac viability at both temperatures. However, this trend was not noted in sour cherry genotypes. Our histological data from autochthonous sweet and sour cherry genotypes and other pomological studies will support future breeding efforts with these genotypes.