<p>The dry roots and rhizomes of <i>Salvia miltiorrhiza</i> are widely used for the treatment of cardiovascular diseases, but the current yield and content of medicinal ingredients cannot meet market demand. Hybridization is a classic method for creating high yield and high-quality materials. However, the hybrid breeding of <i>S. miltiorrhiza</i> is relatively lagging, mainly due to the lack of research data on related <i>S. miltiorrhiza</i> hybrid materials. In this study, 46 hybrid materials obtained through hybridization between <i>S. miltiorrhiza</i> accessions from Sichuan, Shandong, and Henan provinces were evaluated. In 2020 and 2021, fresh weight, tanshinones content, salvianolic acid B content, and four root agronomic traits were measured. The results indicate significant differences among the F₁ materials. Four excellent new materials that meet the requirements of the Pharmacopoeia of the People's Republic of China (2020 edition, volume 1) were selected through principal component analysis. Among them, H9 and H12 exhibit high-yield germplasm resources, while material D11 has the highest tanshinone content and material D14 has the highest salvianolic acid B content. Three consecutive growing seasons (2022–2024) demonstrated consistent expression of superior traits in the four materials, which significantly outperformed the control cultivar CDS-2. These newly created materials can provide excellent germplasm resources for the production and variety improvement of <i>S. miltiorrhiza</i>.</p>