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<p>Root-knot nematodes (RKNs; <i>Meloidogyne</i> spp.) are serious soil pests that affect crops. The pepper accession line ‘LS2341’ is recognised in Japan for its resistance to RKNs and is used in breeding rootstock varieties of peppers (<i>Capsicum annuum</i>). However, the characteristics of the resistance gene remain unclear. Therefore, in this study, we analysed how the ‘LS2341’ line inherits its resistance to <i>Meloidogyne incognita</i>, a species of RKNs. We genetically mapped the resistance gene using the available doubled haploid (DH) population, KL-DH, which displayed segregating resistance phenotypes from the ‘LS2341’ line and 597 PCR-based DNA markers. The resistance gene, designated <i>Mi-nishi</i>, was inherited in a monogenic manner and was mapped to linkage group (LG) 9 (chromosome 9). In addition, allelic tests using LC-DH, a DH population derived from a cross between ‘LS2341’ and ‘CM334’, harbouring the known resistance gene <i>Me7</i>, showed that all 92 lines tested showed resistance, indicating that <i>Mi-nishi</i> may also be an allele of <i>Me7</i>. Because the markers used for the KL-DH map were anchored to the tomato genome, we further developed new single nucleotide polymorphism or insertion/deletion polymorphism markers using the synteny relationships with tomato and reconstructed LG9. Finally, the <i>Mi-nishi</i> gene was mapped between the two DNA markers at a genetic distance of 4&#xa0;cM. The adjacent DNA markers developed in this study will contribute significantly to marker-assisted selection using the ‘LS2341’ line.</p>

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Detailed mapping of a single dominant resistance gene, Mi-nishi, against Meloidogyne incognita from the ‘LS2341’ line of pepper (Capsicum annuum)

  • 期刊论文

  • 发表日期：

    2025年6月5日

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  • Akio Ohyama,
  • Toru Sugita,
  • Hideaki Iwahori,
  • Yuki Maehata,
  • Hiroyuki Fukuoka

摘要

Root-knot nematodes (RKNs; Meloidogyne spp.) are serious soil pests that affect crops. The pepper accession line ‘LS2341’ is recognised in Japan for its resistance to RKNs and is used in breeding rootstock varieties of peppers (Capsicum annuum). However, the characteristics of the resistance gene remain unclear. Therefore, in this study, we analysed how the ‘LS2341’ line inherits its resistance to Meloidogyne incognita, a species of RKNs. We genetically mapped the resistance gene using the available doubled haploid (DH) population, KL-DH, which displayed segregating resistance phenotypes from the ‘LS2341’ line and 597 PCR-based DNA markers. The resistance gene, designated Mi-nishi, was inherited in a monogenic manner and was mapped to linkage group (LG) 9 (chromosome 9). In addition, allelic tests using LC-DH, a DH population derived from a cross between ‘LS2341’ and ‘CM334’, harbouring the known resistance gene Me7, showed that all 92 lines tested showed resistance, indicating that Mi-nishi may also be an allele of Me7. Because the markers used for the KL-DH map were anchored to the tomato genome, we further developed new single nucleotide polymorphism or insertion/deletion polymorphism markers using the synteny relationships with tomato and reconstructed LG9. Finally, the Mi-nishi gene was mapped between the two DNA markers at a genetic distance of 4 cM. The adjacent DNA markers developed in this study will contribute significantly to marker-assisted selection using the ‘LS2341’ line.