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<p>Common bean is one of the main legume crops for human consumption. Drought is the most important abiotic stress factor that limits the growth of common beans. Common bean drought tolerance is a complicated characteristic regulated by numerous genes. Screening drought-tolerance genes and analyzing their regulatory mechanisms are the basis of accurately breeding drought-tolerance varieties. In the present study, we used whole transcriptome RNA sequence to analyze the dynamic transcriptome changes of common bean seedlings under drought stress (DS, treated with 50&#xa0;mmol·L<sup>−1</sup> mannitol for 24&#xa0;h and 48&#xa0;h). Under the conditions of DS for 24&#xa0;h and 48&#xa0;h, 3897 and 5222 differentially expressed (DE) mRNAs, 695 and 883 DE long non-coding RNAs (lncRNAs), 20 and 61 DE microRNAs (miRNAs), 4 and 5 DE circular RNAs (circRNAs) were identified, respectively. The regulatory function under DS was shown by creating a competitive endogenous RNA (ceRNA) network utilizing the predicted DE pairings. Functional analysis showed that ceRNAs were related to polygalacturonase activity, sterol biosynthesis, and the transduction of plant hormone signals. In conclusion, we conducted the first full transcriptome analysis of the common bean under DS, providing a basis for studying the potential function of lncRNA and circRNA.</p>

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Whole-transcriptome RNA sequencing reveals the global molecular responses in response to drought stress in common bean (Phaseolus vulgaris L.)

  • 期刊论文

  • 发表日期：

    2025年5月13日

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  • Debao Cai,
  • Yupeng Zhang,
  • Donghui Ding,
  • Shuqiong Yang,
  • Jun Zhang,
  • Jibao Chen

摘要

Common bean is one of the main legume crops for human consumption. Drought is the most important abiotic stress factor that limits the growth of common beans. Common bean drought tolerance is a complicated characteristic regulated by numerous genes. Screening drought-tolerance genes and analyzing their regulatory mechanisms are the basis of accurately breeding drought-tolerance varieties. In the present study, we used whole transcriptome RNA sequence to analyze the dynamic transcriptome changes of common bean seedlings under drought stress (DS, treated with 50 mmol·L−1 mannitol for 24 h and 48 h). Under the conditions of DS for 24 h and 48 h, 3897 and 5222 differentially expressed (DE) mRNAs, 695 and 883 DE long non-coding RNAs (lncRNAs), 20 and 61 DE microRNAs (miRNAs), 4 and 5 DE circular RNAs (circRNAs) were identified, respectively. The regulatory function under DS was shown by creating a competitive endogenous RNA (ceRNA) network utilizing the predicted DE pairings. Functional analysis showed that ceRNAs were related to polygalacturonase activity, sterol biosynthesis, and the transduction of plant hormone signals. In conclusion, we conducted the first full transcriptome analysis of the common bean under DS, providing a basis for studying the potential function of lncRNA and circRNA.