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<p>Melon leaves are important vegetative organs and main organs for photosynthesis. Leaf shape and structure play a crucial role in determining crop productivity and biomass, making leaf morphology a significant agronomic characteristic. However, the molecular mechanisms underlying the regulation of melon leaf shape are still unclear. Genetic analysis of the F<sub>2</sub> population from the cross between ‘1310’ (normal leaf) and ‘145 × 1901’ (lobed leaf) showed that the variation in melon leaf lobes was regulated by dominant epistasis genes. Bulk segregation analysis-sequencing (BSA-seq) was conducted using two melon inbred lines and their F<sub>2</sub> populations. A 1.59&#xa0;Mb long candidate region (comprising 175 genes and 1754 markers) on chromosome 2 was found to be associated with the melon leaf lobe trait. Further, the BSA-seq results were combined with RNA-sequencing (RNA-seq) for enrichment and expression analyses of the candidate genes. This approach led to the identification of 38 key differentially expressed genes (DEGs). Of these, seven candidate genes were subjected to real-time-quantitative polymerase chain reaction (RT-qPCR) and demonstrated differential expression patterns between normal and lobed leaves. By fine investigating of SNP loci, the candidate region was narrowed to 100&#xa0;kb, which comprised one DEG, a non-synonymous mutation gene <i>MELO3C015426</i>. Based on gene annotation and expression analysis, <i>MELO3C015426</i> (encoding a leucine-rich repeat (LRR) protein) was considered to be the most crucial candidate gene. Kompetitive Allele-Specific PCR (KASP) primers were designed based on the SNP variations of this gene and could be used to screen for high-quality lobed-leaf lines and support molecular marker-assisted breeding.</p>

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Mapping of leaf lobe-related genes in melon (Cucumis melo L.) via bulked segregant analysis

  • 期刊论文

  • 发表日期：

    2025年2月3日

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  • Meng-Ling Zheng,
  • Lu Yang,
  • Ding-Ding Zuo,
  • Rui Sun,
  • Jing Zhang,
  • Yan-Ge Li,
  • Zhong-Hou Zhu,
  • Xue-Jie Zhu,
  • Da-Long Guo

摘要

Melon leaves are important vegetative organs and main organs for photosynthesis. Leaf shape and structure play a crucial role in determining crop productivity and biomass, making leaf morphology a significant agronomic characteristic. However, the molecular mechanisms underlying the regulation of melon leaf shape are still unclear. Genetic analysis of the F2 population from the cross between ‘1310’ (normal leaf) and ‘145 × 1901’ (lobed leaf) showed that the variation in melon leaf lobes was regulated by dominant epistasis genes. Bulk segregation analysis-sequencing (BSA-seq) was conducted using two melon inbred lines and their F2 populations. A 1.59 Mb long candidate region (comprising 175 genes and 1754 markers) on chromosome 2 was found to be associated with the melon leaf lobe trait. Further, the BSA-seq results were combined with RNA-sequencing (RNA-seq) for enrichment and expression analyses of the candidate genes. This approach led to the identification of 38 key differentially expressed genes (DEGs). Of these, seven candidate genes were subjected to real-time-quantitative polymerase chain reaction (RT-qPCR) and demonstrated differential expression patterns between normal and lobed leaves. By fine investigating of SNP loci, the candidate region was narrowed to 100 kb, which comprised one DEG, a non-synonymous mutation gene MELO3C015426. Based on gene annotation and expression analysis, MELO3C015426 (encoding a leucine-rich repeat (LRR) protein) was considered to be the most crucial candidate gene. Kompetitive Allele-Specific PCR (KASP) primers were designed based on the SNP variations of this gene and could be used to screen for high-quality lobed-leaf lines and support molecular marker-assisted breeding.