<p>Microsphere carbon was prepared from used plastic water bottles (PET). The main objective of the present paper is to examine the effectiveness of microsphere carbon synthesized from waste PET plastic bottle with respect to seed germination, growth and development. The synthesized microsphere carbon was characterized by various analytic instruments such as SEM–EDX, XRD and FTIR and finally the synthesized microsphere carbon was applied for their application in the field of germination of <i>Cicer arietinum</i> and biochemical analysis such as pigment contents (chlorophyll ‘a’, ‘b’ and total chlorophyll), carbohydrate, protein and ascorbic acid, amino acid, proline and MDA level, catalase along with germination, morpho-physiological parameters such root and shoot length, fresh and dry biomass, root morphology and cell death. The characterization by SEM and XRD results revealed that PET plastics were comfortably converted to microsphere carbon with a diameter range between 2–8&#xa0;µm with amorphous nature and FTIR study suggested the existence of aromatic C-H and aromatic ring along with carbonyl groups. Similarly, the status of root morphology was also suggested that both root length and seminal root number were gradually decreased with increasing the dose of microsphere carbon dose. Biochemical results revealed that the level of proline, catalase and MDA levels significantly (<i>p</i> &lt; 0.0001) increase with increasing the dose of microsphere carbon. Finally, it can be concluded that lower dose (100&#xa0;mg/L) of microsphere carbon could be beneficial for both seed germination and seedling growth, but higher dose may have adverse effect on plant community.</p>