<p>The two-step steam explosion (TSSE) technology facilitates the effective hydrolysis of cellulose and hemicellulose in lignocellulosic materials, converting them into C5 and C6 sugars. This study concentrates on the production of C6 sugars using an engineered experimental setup, involving precise calculations of experimental data and the application of Statistical Analysis System (SAS) software for mathematical modeling and analysis. The aim is to ascertain the optimal process parameters for industrial-scale production, fulfilling the prerequisites for subsequent engineering design and practical application. During the TSSE process, the initial stage employs relatively moderate reaction conditions, characterized by lower steam temperature and pressure within the reactor and an extended reaction period. Its main purpose is to decompose hemicellulose and generate pentose sugars. Following this, the filter residue from the initial stage undergoes a second steam explosion, where reaction conditions intensify, featuring higher steam temperature and pressure in the reactor and a reduced reaction period. This stage predominantly breaks down cellulose to produce hexose sugars. This study performed a comparative analysis of the process conditions for the two-stage steam explosion and fine-tuned critical parameters such as steam temperature, pressure, and residence time in the reactor, based on the material composition and desired products. By examining the impact of various parameters in the two-stage steam explosion on the yields of pentose and hexose sugars, regression equations were formulated using SAS software, capturing the relationship between sugar yield and process parameters. Through factor analysis, the key process parameters influencing the experimental outcomes were ultimately determined.</p>