Scholarlink

<p>Natural rubber (NR) is a vital industrial raw material currently produced almost exclusively from <i>Hevea brasiliensis</i>. The increasing global demand for NR, combined with the vulnerability of rubber tree plantations to devastating diseases such as South American Leaf Blight, has intensified the search for alternative rubber-producing crops. <i>Taraxacum kok-saghyz</i> is considered one of the most promising alternatives; however, its cultivation remains constrained by several challenges, including susceptibility to root-associated diseases. The aim of this study was to identify and characterize bacterial agents responsible for root rot in <i>T. kok-saghyz</i> cultivated under aeroponic phytotron and <i>in vitro</i> conditions, as well as to assess their effects on plant growth and development in vegetation vessels. Bacterial isolates were recovered from root samples and identified using biochemical characterization together with molecular biological analyses based on the 16S rDNA, <i>gyrB</i>, and <i>rpoD</i> sequencing. Pathogenicity was evaluated through inoculation of healthy <i>in vitro</i>-grown plants and hairy root (HR) cultures. Two bacterial species, <i>Pseudomonas putida</i> and <i>Raoultella terrigena</i>, were identified as causative agents of root rot. Healthy <i>in vitro</i>-grown plants inoculated with <i>P. putida</i> and <i>R. terrigena</i> exhibited progressive disease symptom development and deterioration. By contrast, plants derived from pathogen-free disinfected seeds demonstrated stable growth, tolerance to elevated summer temperatures, and a significant increase in biomass accumulation. Although <i>P. putida</i> and <i>R. terrigena</i> are commonly described as endophytic microorganisms, our findings demonstrate that under specific conditions they may exhibit pathogenic behavior. Thus, interactions between <i>T. kok-saghyz</i> and its associated microorganisms are strongly influenced by environmental and cultivation conditions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification and pathogenicity of Raoultella and Pseudomonas species associated with roots of Taraxacum kok-saghyz

  • 期刊论文

  • 发表日期：

    2026年7月14日

查看全文
  • Levon Y. Martirosyan,
  • Valentina V. Martirosyan,
  • Yuriy A. Rybakov,
  • Alexander Y. Amerik,
  • Yuriy T. Martirosyan

摘要

Natural rubber (NR) is a vital industrial raw material currently produced almost exclusively from Hevea brasiliensis. The increasing global demand for NR, combined with the vulnerability of rubber tree plantations to devastating diseases such as South American Leaf Blight, has intensified the search for alternative rubber-producing crops. Taraxacum kok-saghyz is considered one of the most promising alternatives; however, its cultivation remains constrained by several challenges, including susceptibility to root-associated diseases. The aim of this study was to identify and characterize bacterial agents responsible for root rot in T. kok-saghyz cultivated under aeroponic phytotron and in vitro conditions, as well as to assess their effects on plant growth and development in vegetation vessels. Bacterial isolates were recovered from root samples and identified using biochemical characterization together with molecular biological analyses based on the 16S rDNA, gyrB, and rpoD sequencing. Pathogenicity was evaluated through inoculation of healthy in vitro-grown plants and hairy root (HR) cultures. Two bacterial species, Pseudomonas putida and Raoultella terrigena, were identified as causative agents of root rot. Healthy in vitro-grown plants inoculated with P. putida and R. terrigena exhibited progressive disease symptom development and deterioration. By contrast, plants derived from pathogen-free disinfected seeds demonstrated stable growth, tolerance to elevated summer temperatures, and a significant increase in biomass accumulation. Although P. putida and R. terrigena are commonly described as endophytic microorganisms, our findings demonstrate that under specific conditions they may exhibit pathogenic behavior. Thus, interactions between T. kok-saghyz and its associated microorganisms are strongly influenced by environmental and cultivation conditions.