Scholarlink

<p>Severe dieback symptoms have been consistently observed on plants of <i>Pelargonium graveolens</i> L’Her (rose-scented geranium) in the regions of northern India during rainy seasons with hot weather. The initial symptoms observed were yellowing in leaves, shoot blight leading to stem necrosis, defoliation and progressive plant death. The isolated fungus produced greyish-brown to blackish aerial mycelia on potato dextrose agar and exhibited morphological characteristics consistent with <i>Lasiodiplodia</i> species. Pathogenicity assays using a representative isolate reproduced dieback symptoms on healthy geranium plantlets, and the pathogen was successfully re-isolated, fulfilling Koch’s postulates. Molecular identification based on internal transcribed spacer (ITS) and translation elongation factor 1-α (<i>tef1</i>-α) gene sequences confirmed the pathogen as <i>Lasiodiplodia theobromae</i>. Phylogenetic analysis using concatenated ITS and <i>tef</i>1-α sequences placed the isolate within the <i>L. theobromae</i> clade with strong bootstrap support. To the best of our knowledge, this study represents the first report of <i>L. theobromae</i> causing dieback disease in <i>P. graveolens</i> in India.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

First report of Lasiodiplodia theobromae causing dieback disease of Pelargonium graveolens in India

  • 期刊论文

  • 发表日期：

    2026年5月4日

查看全文
  • Bhanu Sharma,
  • Anuj Kumar,
  • Amit Kumar Sinha,
  • Saudan Singh,
  • Kishore Babu Bandamaravuri

摘要

Severe dieback symptoms have been consistently observed on plants of Pelargonium graveolens L’Her (rose-scented geranium) in the regions of northern India during rainy seasons with hot weather. The initial symptoms observed were yellowing in leaves, shoot blight leading to stem necrosis, defoliation and progressive plant death. The isolated fungus produced greyish-brown to blackish aerial mycelia on potato dextrose agar and exhibited morphological characteristics consistent with Lasiodiplodia species. Pathogenicity assays using a representative isolate reproduced dieback symptoms on healthy geranium plantlets, and the pathogen was successfully re-isolated, fulfilling Koch’s postulates. Molecular identification based on internal transcribed spacer (ITS) and translation elongation factor 1-α (tef1-α) gene sequences confirmed the pathogen as Lasiodiplodia theobromae. Phylogenetic analysis using concatenated ITS and tef1-α sequences placed the isolate within the L. theobromae clade with strong bootstrap support. To the best of our knowledge, this study represents the first report of L. theobromae causing dieback disease in P. graveolens in India.