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<p>Grapevine trunk diseases (GTDs) have resulted in huge economic losses in grapevine production worldwide, including in Iran. <i>Fomitiporia mediterranea</i> (M. Fisch.) is one of the primary causal fungi of GTDs, attacking the wood and causing a white rot that gradually develops and obstructs essential water transport. This white rot inflicts greater damage under dry and heat-stress conditions, making the disease particularly challenging to manage. The antagonistic isolate <i>Pseudomonas fluorescens</i> GI310 was used to produce crude lipopeptide (LP) extract. The minimum inhibitory concentration (MIC) of the crude lipopeptide extract of <i>P. fluorescens</i> GI310 against <i>F. mediterranea</i> was determined to be 25&#xa0;µg/mL. The crude extract of lipopeptide was fractionated by column chromatography. Two fractions displayed antifungal activity against <i>F. mediterranea </i>in vitro<i>.</i> The molecular mass of those fractions was determined with high-resolution mass spectrometry. The mass range was 650–1750&#xa0;m<i>/z</i>. By comparing the results with the PubChem library data, Bananamide 2 and Bananamide 1 were the candidate lipopeptides. This study demonstrates that endophytic bacteria and their metabolites could provide sustainable management of GTDs. This finding may help to achieve the goal of using LPs of endophytic bacteria in vineyards to combat the fungi responsible for grapevine trunk diseases.</p>

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Extraction and identification of antagonistic lipopeptides from Pseudomonas fluorescens GI310 against Fomitiporia mediterranea as a grapevine trunk fungal pathogen

  • 期刊论文

  • 发表日期：

    2025年12月11日

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  • Seyedeh Elham Vaghari Souran,
  • Azam Shekariesfahlan,
  • Maryam Kharatha,
  • Mohsen Farhadpour,
  • Mahdi Moridi Farimani,
  • Abolghasem Ghasemi,
  • Shahram Naeimi

摘要

Grapevine trunk diseases (GTDs) have resulted in huge economic losses in grapevine production worldwide, including in Iran. Fomitiporia mediterranea (M. Fisch.) is one of the primary causal fungi of GTDs, attacking the wood and causing a white rot that gradually develops and obstructs essential water transport. This white rot inflicts greater damage under dry and heat-stress conditions, making the disease particularly challenging to manage. The antagonistic isolate Pseudomonas fluorescens GI310 was used to produce crude lipopeptide (LP) extract. The minimum inhibitory concentration (MIC) of the crude lipopeptide extract of P. fluorescens GI310 against F. mediterranea was determined to be 25 µg/mL. The crude extract of lipopeptide was fractionated by column chromatography. Two fractions displayed antifungal activity against F. mediterranea in vitro. The molecular mass of those fractions was determined with high-resolution mass spectrometry. The mass range was 650–1750 m/z. By comparing the results with the PubChem library data, Bananamide 2 and Bananamide 1 were the candidate lipopeptides. This study demonstrates that endophytic bacteria and their metabolites could provide sustainable management of GTDs. This finding may help to achieve the goal of using LPs of endophytic bacteria in vineyards to combat the fungi responsible for grapevine trunk diseases.