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<p>GDSL lipases, a new-type of hydrolytic enzymes, are involved in multiple biological processes in eukaryotes and prokaryotes. However, little is known about the roles of GDSL lipases in <i>Sclerotinia sclerotiorum</i> (<i>S</i>. <i>sclerotiorum</i>), a worldwide soil-borne phytopathogenic&#xa0;fungus. A lipase-GDSL-domain protein (APA05712.1) of <i>S</i>. <i>sclerotiorum</i>, named SsGLIP1, was shown to play a role in diverse developmental processes and fungal pathogenicity via gene knockout. Compared with the wild-type (WT) strain, the <i>Ssglip1</i> knockout mutant presented slower mycelial growth and non-sclerotia formation. Moreover, the mutant did not form an infection cushion. Infection assays of detached rape leaves revealed that SsGLIP1 was required for full virulence of <i>S</i>. <i>sclerotiorum</i> during infection of susceptible host plants. In addition, the mutant strains showed decreased tolerance to osmotic stress and H<sub>2</sub>O<sub>2</sub>, and did not maintain the integrity of the cell wall and membrane. Taken together, the results of this work revealed that SsGLIP1 plays an essential role in sclerotia formation and virulence, and lay a foundation for revealing the molecular mechanism involved in <i>S</i>. <i>sclerotiorum</i>.</p>

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A GDSL-lipase,SsGLIP1, is involved in regulating the sclerotia formation and virulence of Sclerotinia sclerotiorum

  • 期刊论文

  • 发表日期：

    2025年9月18日

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  • Yilan Qiu,
  • Yi Gao,
  • Jinlian Hu,
  • Yaxin Qi,
  • Shaoqi Wei,
  • Xuemei Kuang,
  • Hui Li

摘要

GDSL lipases, a new-type of hydrolytic enzymes, are involved in multiple biological processes in eukaryotes and prokaryotes. However, little is known about the roles of GDSL lipases in Sclerotinia sclerotiorum (S. sclerotiorum), a worldwide soil-borne phytopathogenic fungus. A lipase-GDSL-domain protein (APA05712.1) of S. sclerotiorum, named SsGLIP1, was shown to play a role in diverse developmental processes and fungal pathogenicity via gene knockout. Compared with the wild-type (WT) strain, the Ssglip1 knockout mutant presented slower mycelial growth and non-sclerotia formation. Moreover, the mutant did not form an infection cushion. Infection assays of detached rape leaves revealed that SsGLIP1 was required for full virulence of S. sclerotiorum during infection of susceptible host plants. In addition, the mutant strains showed decreased tolerance to osmotic stress and H2O2, and did not maintain the integrity of the cell wall and membrane. Taken together, the results of this work revealed that SsGLIP1 plays an essential role in sclerotia formation and virulence, and lay a foundation for revealing the molecular mechanism involved in S. sclerotiorum.