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<p>Bacterial canker of stone fruits caused by <i>Pseudomonas syringae</i> pv<i>. syringae</i> (Pss) is one of the most important diseases of fruit trees worldwide. Despite its significant economic impact, chemical control methods using copper compounds often remain ineffective. Biological control provides a powerful and environmentally friendly alternative to synthetic pesticides. This study sought to isolate and identify epiphyte bacteria from the tissue surface of leaves and shoots of stone trees and investigate their antagonistic effects against pathogenic Pss in laboratory and greenhouse conditions. <i>In vitro</i> analysis of the antagonistic activity of 122 epiphytic bacterial strains showed that 36.78% of the strains could produce an inhibition zone diameter from 20 to 50&#xa0;mm towards Pss. Also, some strains produced antibiotic and volatile organic antimicrobial compounds. The antagonist strains were identified as <i>Pseudomonas</i> sp., <i>Acinetobacter</i> sp., <i>Stenotrophomonas</i> sp. and <i>Pantoea agglomerans</i> due to the physiological and biochemical characteristics and the partial 16S&#xa0;rRNA gene sequence. <i>In vivo</i> tests, the antagonistic <i>Pseudomonas</i> sp. strain 145c and <i>Acinetobacter</i> sp. strain 147b reduced the disease severity of bacterial canker of stone fruit on two-year-old peach trees by 94.80% compared to the positive control. This study demonstrates that epiphytic bacteria from stone fruit trees exhibit strong potential for the control of Pss.</p>

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Exploration of epiphytic bacteria of stone fruit trees for the biocontrol of Pseudomonas syringae pv. syringae

  • 期刊论文

  • 发表日期：

    2025年7月31日

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  • Sepideh Mehrfar,
  • Fatemeh Shahryari,
  • Nargues Falahi Charkhabi

摘要

Bacterial canker of stone fruits caused by Pseudomonas syringae pv. syringae (Pss) is one of the most important diseases of fruit trees worldwide. Despite its significant economic impact, chemical control methods using copper compounds often remain ineffective. Biological control provides a powerful and environmentally friendly alternative to synthetic pesticides. This study sought to isolate and identify epiphyte bacteria from the tissue surface of leaves and shoots of stone trees and investigate their antagonistic effects against pathogenic Pss in laboratory and greenhouse conditions. In vitro analysis of the antagonistic activity of 122 epiphytic bacterial strains showed that 36.78% of the strains could produce an inhibition zone diameter from 20 to 50 mm towards Pss. Also, some strains produced antibiotic and volatile organic antimicrobial compounds. The antagonist strains were identified as Pseudomonas sp., Acinetobacter sp., Stenotrophomonas sp. and Pantoea agglomerans due to the physiological and biochemical characteristics and the partial 16S rRNA gene sequence. In vivo tests, the antagonistic Pseudomonas sp. strain 145c and Acinetobacter sp. strain 147b reduced the disease severity of bacterial canker of stone fruit on two-year-old peach trees by 94.80% compared to the positive control. This study demonstrates that epiphytic bacteria from stone fruit trees exhibit strong potential for the control of Pss.