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<p><i>Clonostachys rosea</i> is a promising fungal biocontrol agent with broad antagonistic activity against plant pathogens, particularly <i>Fusarium</i> species. This study aimed to characterize <i>Fusarium</i> spp. isolated from wilt-infected ridge gourd plants and evaluate the biocontrol efficacy of <i>C. rosea</i> against these pathogens. Twelve <i>Fusarium</i> isolates were identified using morphological and molecular methods, revealed nine <i>Fusarium oxysporum</i>, two <i>F. brachygibbosum</i>, and one <i>F. equiseti</i>. Pathogenicity tests revealed that <i>F. oxysporum</i> isolates were highly virulent, whereas the other two species had milder symptoms. Antagonistic assays showed that <i>C. rosea</i> effectively inhibited the growth of <i>Fusarium</i> species by up to 72.2%. Microscopic examination indicated mycoparasitic activity involved hyphal encirclement and degradation of <i>Fusarium</i> hyphae. GC–MS metabolite profiling identified 44 bioactive compounds, including fatty acids, alcohols, amines, thiols, triazoles and hydrazines. Molecular docking studies demonstrated strong binding affinities of these metabolites to key fungal proteins, suggesting potential antifungal mechanisms. Overall, the findings highlights the potential of <i>C. rosea</i> and its metabolites offering new strategies for managing Fusarium wilt in ridge gourd.</p>

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Unveiling the antifungal potential of Clonostachys rosea against Fusarium wilt: an in vitro and in silico analysis

  • 期刊论文

  • 发表日期：

    2025年5月28日

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  • Vyamasani Shravani,
  • Johnson Iruthayasamy,
  • Saranya Nallusamy,
  • Jothi Govindasamy,
  • Kokiladevi Eswaran,
  • Shanthi Annaiyan

摘要

Clonostachys rosea is a promising fungal biocontrol agent with broad antagonistic activity against plant pathogens, particularly Fusarium species. This study aimed to characterize Fusarium spp. isolated from wilt-infected ridge gourd plants and evaluate the biocontrol efficacy of C. rosea against these pathogens. Twelve Fusarium isolates were identified using morphological and molecular methods, revealed nine Fusarium oxysporum, two F. brachygibbosum, and one F. equiseti. Pathogenicity tests revealed that F. oxysporum isolates were highly virulent, whereas the other two species had milder symptoms. Antagonistic assays showed that C. rosea effectively inhibited the growth of Fusarium species by up to 72.2%. Microscopic examination indicated mycoparasitic activity involved hyphal encirclement and degradation of Fusarium hyphae. GC–MS metabolite profiling identified 44 bioactive compounds, including fatty acids, alcohols, amines, thiols, triazoles and hydrazines. Molecular docking studies demonstrated strong binding affinities of these metabolites to key fungal proteins, suggesting potential antifungal mechanisms. Overall, the findings highlights the potential of C. rosea and its metabolites offering new strategies for managing Fusarium wilt in ridge gourd.