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<p><i>Musa rubra,</i> a dwarf ornamental <i>Musa</i> species, exhibits natural resistance against various biotic and abiotic stresses. This is presumed to be the key gene source for resistance to <i>Fusarium oxysporum</i> f.sp. <i>cubense</i> tropical race 4 (<i>Foc</i> TR4) (VCG 01213/16) which is emerging as a serious threat to banana cultivation across the globe. Therefore, this valuable gene needs to be introgressed into commercial cultivars through breeding, for which uniform and quality planting material of <i>M. rubra</i> is a pre-requisite. Hence, the initial focus of the present study is to optimize the micropropagation protocol for plant regeneration using shoot tip explants. Murashige and Skoog (MS) medium fortified with 6-benzylaminopurine (BAP), thidiazuron (TDZ) and indole-3-acetic acid (IAA) in different combinations was assessed to evaluate which treatment produced the maximum number of plantlets in the shortest time. Subsequently, Inter-Simple Sequence Repeat (ISSR) markers were used to assess potential genetic alterations induced in micropropagated plants under the influence of novel plant growth regulators (PGRs) such as TDZ. Remarkably, the amplified products of the regenerated plants displayed a banding pattern similar to that of the sucker-derived mother plant, affirming the genetic uniformity of the micropropagated plantlets. Furthermore, the inherent resistance of <i>M. rubra</i> plantlets against <i>Fusarium oxysporum</i> f. sp<i>. cubense</i> tropical race 4 (<i>Foc</i> TR4) (VCG 01213/16) was confirmed through pot culture screening of the shoot tip derived plants of <i>M. rubra.</i></p>

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Ensuring genetic uniformity and fusarium wilt (TR4) resistance in shoot tip derived plantlets of wild species M. rubra

  • 期刊论文

  • 发表日期：

    2025年3月12日

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  • Saraswathi Marimuthu Somasundaram,
  • Mahendran Jayakumar,
  • Sharmila Gayatri Durairajan,
  • Thangavelu Raman,
  • Salini Arumugam Palanivelu,
  • Karthi Chinnadurai,
  • Bathrinath Murugesan,
  • Umabharathi Murugesan,
  • Uma Subbaraya

摘要

Musa rubra, a dwarf ornamental Musa species, exhibits natural resistance against various biotic and abiotic stresses. This is presumed to be the key gene source for resistance to Fusarium oxysporum f.sp. cubense tropical race 4 (Foc TR4) (VCG 01213/16) which is emerging as a serious threat to banana cultivation across the globe. Therefore, this valuable gene needs to be introgressed into commercial cultivars through breeding, for which uniform and quality planting material of M. rubra is a pre-requisite. Hence, the initial focus of the present study is to optimize the micropropagation protocol for plant regeneration using shoot tip explants. Murashige and Skoog (MS) medium fortified with 6-benzylaminopurine (BAP), thidiazuron (TDZ) and indole-3-acetic acid (IAA) in different combinations was assessed to evaluate which treatment produced the maximum number of plantlets in the shortest time. Subsequently, Inter-Simple Sequence Repeat (ISSR) markers were used to assess potential genetic alterations induced in micropropagated plants under the influence of novel plant growth regulators (PGRs) such as TDZ. Remarkably, the amplified products of the regenerated plants displayed a banding pattern similar to that of the sucker-derived mother plant, affirming the genetic uniformity of the micropropagated plantlets. Furthermore, the inherent resistance of M. rubra plantlets against Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) (VCG 01213/16) was confirmed through pot culture screening of the shoot tip derived plants of M. rubra.