<p>The Pantanal is one of the largest wetlands in the world and is of great importance for biodiversity and the socioeconomics of central South America. Nevertheless, the Pantanal is currently facing severe anthropogenic impacts, including chemical disturbances. Herein we quantified the levels of arsenic (As), silver (Ag), aluminum (Al), barium (Ba), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), mercury (Hg), manganese (Mn), molybdenum (Mo), lead (Pb), selenium (Se), vanadium (V), and zinc (Zn) in caudal tips of snakes from Southern Pantanal. Except for Zn, an essential element naturally found in elevated concentrations in snakes and other vertebrates, the highest levels were found for Al, Ba, and Mn. Notably, impure iron and manganiferous ores intensively explored in the studied region present elevated Al, Ba, and Mn content. Concentrations of As were positively correlated to the size of the yellow anaconda <i>Eunectes notaeus</i> while Ba levels were negatively correlated to size in this species. Levels of Mn and V were negatively correlated with the size of the water snake <i>Helicops leopardinus</i>. Higher burdens in species that feed on higher trophic levels were not verified for any element. Significant intraspecific differences in species commonly sampled in two sites with distinct degrees of anthropogenic disturbance were detected only for the yellow-bellied snake <i>Erythrolamprus poecilogyrus</i>. Our study suggests that mining activity could lead to mobilization and bioaccumulation of metals and metalloids in snakes from Southern Pantanal, but allochthonous sources from agricultural, silvicultural, and urban areas from the adjacent Brazilian Central Plateau should also be considered.</p>